[Leu27] insulin-like growth factor II is highly selective for the type-II IGF receptor in binding, cross-linking and thymidine incorporation experiments

Margot W. Beukers, Youngman Oh, Heping Zhang, Nicholas Ling, Ron G. Rosenfeld

Research output: Research - peer-reviewArticle

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Abstract

Insulin-like growth factor-II (IGF-II) is able to interact with three different receptors: the type-I and type-II IGF receptors, and the insulin receptor, although with a lower affinity. This cross reactivity obscures the mechanisms via which the biological activities of IGF-II are mediated. We have synthesized an IGF-II analog, [Leu27] IGF-II, that is highly selective for the type-II IGF receptor. Receptor binding experiments demonstrate a high affinity for the type-II IGF receptor, analogous to synthetic (syn) and recombinant (rec) IGF-II, but no affinity for the type-I IGF and the insulin receptor at concentrations up to 50 and 200 ng/ ml, respectively. The lack of affinity for these two receptors is confirmed by biological studies which demonstrated that this analog, in contrast with synIGF-II, did not stimulate [3H] thymidine incorporation in Balb/ c 3T3 cells. [Leu27] IGF-II opens new ways to identify which actions of IGF-II are mediated via the type-II receptor and which are due to cross reactivity with the type-I IGF or the insulin receptor.

LanguageEnglish (US)
Pages1201-1203
Number of pages3
JournalEndocrinology
Volume128
Issue number2
StatePublished - Feb 1991
Externally publishedYes

Fingerprint

IGF Type 2 Receptor
Insulin-Like Growth Factor II
Thymidine
Insulin Receptor
3T3 Cells
IGF Type 1 Receptor

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

[Leu27] insulin-like growth factor II is highly selective for the type-II IGF receptor in binding, cross-linking and thymidine incorporation experiments. / Beukers, Margot W.; Oh, Youngman; Zhang, Heping; Ling, Nicholas; Rosenfeld, Ron G.

In: Endocrinology, Vol. 128, No. 2, 02.1991, p. 1201-1203.

Research output: Research - peer-reviewArticle

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