Lectin Receptors of Amyloid in Corneas with Lattice Dystrophy

Noorjahan Panjwani, Kevin Free, Jules Baum, Daniel Albert, Merlyn Rodrigues, Jay H. Krachmer

Research output: Contribution to journalArticle

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Abstract

We analyzed lectin binding patterns of amyloid glycoconjugates in patients with lattice dystrophy of the cornea. Results of paraffin and frozen sections differed in some instances. With paraffin sections, three lectins—wheat germ agglutinin (WGA), Ricinus communis agglutinin I (RCA-I), and concanavalin A (Con A)—stained the abnormal deposits. In frozen sections, the abnormal deposits were stained by five lectins—WGA, RCA-I, Con A, peanut agglutinin (PNA), and soybean agglutinin (SBA). In paraffin sections, PNA and SBA did not stain amyloid deposits. In both paraffin and frozen sections, some lectin-positive deposits corresponded to the Congo red-positive material, whereas others were present surrounding and encroaching on the Congo red-reactive material. This study demonstrates that WGA-, RCA-I-, Con A—, PNA-, and SBA-positive abnormal deposits are present in corneas with lattice dystrophy. Since lectins bind to specific sugar residues, we conclude that the abnormal deposits consist, at least in part, of glycoconjugates and that these glycoconjugates contain oligosaccharides with N-acetylglucosamine/sialic acid, mannose/ glucose and terminal β-galactose residues and chains with terminal β-galactose-N-acetylgalactosamine disaccharides.

Original languageEnglish (US)
Pages (from-to)688-691
Number of pages4
JournalArchives of ophthalmology
Volume105
Issue number5
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

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Mitogen Receptors
Peanut Agglutinin
Amyloid
Paraffin
Cornea
Glycoconjugates
Frozen Sections
Concanavalin A
Lectins
Congo Red
Galactose
Acetylgalactosamine
Acetylglucosamine
Agglutinins
Disaccharides
Amyloid Plaques
N-Acetylneuraminic Acid
Mannose
Oligosaccharides
Coloring Agents

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Lectin Receptors of Amyloid in Corneas with Lattice Dystrophy. / Panjwani, Noorjahan; Free, Kevin; Baum, Jules; Albert, Daniel; Rodrigues, Merlyn; Krachmer, Jay H.

In: Archives of ophthalmology, Vol. 105, No. 5, 01.01.1987, p. 688-691.

Research output: Contribution to journalArticle

Panjwani, N, Free, K, Baum, J, Albert, D, Rodrigues, M & Krachmer, JH 1987, 'Lectin Receptors of Amyloid in Corneas with Lattice Dystrophy', Archives of ophthalmology, vol. 105, no. 5, pp. 688-691. https://doi.org/10.1001/archopht.1987.01060050106047
Panjwani, Noorjahan ; Free, Kevin ; Baum, Jules ; Albert, Daniel ; Rodrigues, Merlyn ; Krachmer, Jay H. / Lectin Receptors of Amyloid in Corneas with Lattice Dystrophy. In: Archives of ophthalmology. 1987 ; Vol. 105, No. 5. pp. 688-691.
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