Lectin-induced regulation of human thymus-independent anti-TNP responses in vitro

B. Golding, D. R. Burger, Marvin Rittenberg

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Recently we showed that human hapten-specific T cell-independent (TI) antibody responses could be elicited in vitro with the antigen trinitrophenylated Brucella abortus (TNP-Ba). Although by definition T cells are not required for TI responses, they have been shown capable of regulating such responses in the mouse. In this study we examine the ability of the T cell lectins Con A and PHA to modulate TI responses of human tonsil cells. Addition of the lectins to cultures on day 0 or 3 resulted in inhibition (> 90%) or enhancement (> 150%) of the anti-TNP PFC response, respectively. The inhibition was only apparent if the lectins were added at the onset of culture but not 24 hr later, and was T cell-dependent, because T cell-depleted cultures (<0.5% E-rosetting cells) could not be inhibited. The enhancement observed was even greater in T cell-depleted cultures and was antigen-specific because TNP-SRBC but not PC-SRBC targets were lysed and the PFC were inhibited by soluble TNP (> 70%). This finding suggested that anti-TNP PFC precursors were proliferating more vigorously or that additional, previously antigen-insensitive cells were being recruited in the presence of lectin.

Original languageEnglish (US)
Pages (from-to)942-945
Number of pages4
JournalJournal of Immunology
Volume129
Issue number3
StatePublished - 1982
Externally publishedYes

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Lectins
Thymus Gland
T-Lymphocytes
Brucella abortus
Antigens
Haptens
Palatine Tonsil
In Vitro Techniques
Antibody Formation
Cell Culture Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Golding, B., Burger, D. R., & Rittenberg, M. (1982). Lectin-induced regulation of human thymus-independent anti-TNP responses in vitro. Journal of Immunology, 129(3), 942-945.

Lectin-induced regulation of human thymus-independent anti-TNP responses in vitro. / Golding, B.; Burger, D. R.; Rittenberg, Marvin.

In: Journal of Immunology, Vol. 129, No. 3, 1982, p. 942-945.

Research output: Contribution to journalArticle

Golding, B, Burger, DR & Rittenberg, M 1982, 'Lectin-induced regulation of human thymus-independent anti-TNP responses in vitro', Journal of Immunology, vol. 129, no. 3, pp. 942-945.
Golding, B. ; Burger, D. R. ; Rittenberg, Marvin. / Lectin-induced regulation of human thymus-independent anti-TNP responses in vitro. In: Journal of Immunology. 1982 ; Vol. 129, No. 3. pp. 942-945.
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