TY - JOUR
T1 - Langerhans cells in acute and chronic epidermal lesions of atopic dermatitis, observed by L-dopa histofluorescence, glycol methacrylate thin section, and electron microscopy
AU - Uno, H.
AU - Hanifin, J. M.
N1 - Funding Information:
Preliminary results were presented at the National Meeting of the American Federation for Clinical Research, Washington, DC, May 1979. Publication No. 1070 of the Oregon Regional Primate Research Center. Supported in part by Grants RR-00163 and RR-05694 from the National Institutes of Health. Reprint requests to: Hideo Uno, M.D., Wisconsin Regional Primate Research Center, 1223 Capitol Ct., Madison, WI 53706.
PY - 1980
Y1 - 1980
N2 - Since epidermal Langerhans cells are known to play an immunopathologic role in allergic skin diseases, we explored the histological features of Langerhans cells associated with epidermal lesions in acute and chronic stages of atopic dermatitis. The L-dopa histofluorescence method, introduced by Falck and others in 1976, has provided a new approach to visualization of Langerhans cells in normal and pathologic skin of both experimental animals and human beings. We used this technique for skin biopsy specimens from normal individuals and patients with atopic dermatitis. We also used embedding of thin sections in glycol methacrylate, electron microscopy, and autoradiography of 3H L-dopa to observe morphological characteristics of Langerhans cells in these skin specimens. We observed a few Langerhans cells in the spongiotic epidermis of acute erythematous lesions, but many fluorescent infiltrating mononuclear cells were also present in both epidermal and subepidermal layers. In chronic lichenified lesions significantly increased numbers of Langerhans cells were distributed throughout acanthotic epidermis, but the numbers of infiltrating mononuclear cells in the epidermis were greatly reduced. Focal accumulations of both Langerhans cells and monocytic cells were occasionally found in the epidermis of chronic lesions; these accumulations are thought to be recurrent inflammatory foci in the chronic lesions. The use of L-dopa histofluorescence to study epidermal Langerhans cells demonstrates Langerhans cells more clearly than other histological means.
AB - Since epidermal Langerhans cells are known to play an immunopathologic role in allergic skin diseases, we explored the histological features of Langerhans cells associated with epidermal lesions in acute and chronic stages of atopic dermatitis. The L-dopa histofluorescence method, introduced by Falck and others in 1976, has provided a new approach to visualization of Langerhans cells in normal and pathologic skin of both experimental animals and human beings. We used this technique for skin biopsy specimens from normal individuals and patients with atopic dermatitis. We also used embedding of thin sections in glycol methacrylate, electron microscopy, and autoradiography of 3H L-dopa to observe morphological characteristics of Langerhans cells in these skin specimens. We observed a few Langerhans cells in the spongiotic epidermis of acute erythematous lesions, but many fluorescent infiltrating mononuclear cells were also present in both epidermal and subepidermal layers. In chronic lichenified lesions significantly increased numbers of Langerhans cells were distributed throughout acanthotic epidermis, but the numbers of infiltrating mononuclear cells in the epidermis were greatly reduced. Focal accumulations of both Langerhans cells and monocytic cells were occasionally found in the epidermis of chronic lesions; these accumulations are thought to be recurrent inflammatory foci in the chronic lesions. The use of L-dopa histofluorescence to study epidermal Langerhans cells demonstrates Langerhans cells more clearly than other histological means.
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U2 - 10.1111/1523-1747.ep12521130
DO - 10.1111/1523-1747.ep12521130
M3 - Article
C2 - 6993585
AN - SCOPUS:0018867706
SN - 0022-202X
VL - 75
SP - 52
EP - 60
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 1
ER -