Lactoferrin binds to cell membrane DNA. Association of surface DNA with an enriched population of B cells and monocytes

Robert (Rob) Bennett, J. Davis, S. Campbell, S. Portnoff

    Research output: Contribution to journalArticle

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    Abstract

    The binding of human 125I-labeled lactoferrin (LF) to a population of adherent mononuclear cells (ADMC) and nonrosetting lymphocytes (E-) was abolished by prior treatment of the cells with deoxyribonuclease (DNase), but not ribonuclease (RNase). When DNase-treated ADMC were incubated with exogenous DNA, the binding of 125I-LF was restored. Enzymatic digestion with other enzymes, trypsin, phospholipase D, and neuraminidase, did not significantly influence 125I-LF binding. Saturable binding of LF at 0°C was demonstrated for both E- and ADMC, with equilibrium dissociation constants of 0.76 x 10-6 M and 1.8 x 10-6 M, respectively. E- cells bound 2.5 x 107 and ADMC bound 3.3 x 107 molecules of LF at saturation. Cell membranes were isolated from ADMC, E- and E+ and reacted with 125I-labeled LF; significant binding was only seen with ADMC and E-. Prior treatment of the membranes with DNase abolished the binding. Immunofluorescence studies indicated that a population of ADMC and E-, but not E+, exhibited a peripheral staining pattern for LF. Prior treatment of ADMC and E- with DNase abolished the surface immunofluorescence. This study provides evidence that cell membrane DNA acts as a binding site for exogenous LF. This is a novel role for DNA that has not been previously reported. Furthermore, it points to a basic difference between E+ cells vs. ADMC and E- cells in respect to their possession of cell surface DNA.

    Original languageEnglish (US)
    Pages (from-to)611-618
    Number of pages8
    JournalJournal of Clinical Investigation
    Volume71
    Issue number3
    StatePublished - 1983

    Fingerprint

    Lactoferrin
    Monocytes
    B-Lymphocytes
    Cell Membrane
    DNA
    Population
    Deoxyribonucleases
    Fluorescent Antibody Technique
    Phospholipase D
    Neuraminidase
    Ribonucleases
    Trypsin
    Digestion

    ASJC Scopus subject areas

    • Medicine(all)

    Cite this

    Lactoferrin binds to cell membrane DNA. Association of surface DNA with an enriched population of B cells and monocytes. / Bennett, Robert (Rob); Davis, J.; Campbell, S.; Portnoff, S.

    In: Journal of Clinical Investigation, Vol. 71, No. 3, 1983, p. 611-618.

    Research output: Contribution to journalArticle

    Bennett, Robert (Rob) ; Davis, J. ; Campbell, S. ; Portnoff, S. / Lactoferrin binds to cell membrane DNA. Association of surface DNA with an enriched population of B cells and monocytes. In: Journal of Clinical Investigation. 1983 ; Vol. 71, No. 3. pp. 611-618.
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