Lack of sex-linked differences in cerebral edema and aquaporin-4 expression after experimental stroke

Xiaoqin Liu, Wenri Zhang, Nabil Alkayed, Stanley C. Froehner, Marvin E. Adams, Mahmood Amiry-Moghaddam, Ole Petter Ottersen, Patricia D. Hurn, Anish Bhardwaj

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Aquaporin-4 (AQP4) has been shown to be important in the evolution of stroke-associated cerebral edema. However, the role of AQP4 in stroke-associated cerebral edema as it pertains to sex has not been previously studied. The perivascular pool of AQP4 is important in the influx and efflux of water during focal cerebral ischemia. We used mice with targeted disruption of the gene encoding α-syntrophin (α-Syn-/-) that lack the perivascular AQP4 pool but retain the endothelial pool of this protein. Infarct volume at 72 h after transient focal ischemia (90 mins) in isoflurane- anesthetized mice was attenuated in both sexes with α-Syn deletion as compared with their wild-type (WT) counterparts. There were no sex differences in hemispheric water content in WT and α-Syn-/- mice or regional AQP4 expression in WT mice. In neither sex did α-Syn deletion lead to alterations in end-ischemic regional cerebral blood flow (rCBF). These data suggest that after experimental stroke: (1) there is no difference in stroke-associated cerebral edema based on sex, (2) AQP4 does not involve in sex-based differences in stroke volume, and (3) perivascular pool of AQP4 has no significant role in end-ischemic rCBF.

Original languageEnglish (US)
Pages (from-to)1898-1906
Number of pages9
JournalJournal of Cerebral Blood Flow and Metabolism
Volume28
Issue number12
DOIs
StatePublished - Dec 2008

Fingerprint

Aquaporin 4
Brain Edema
Sex Characteristics
Stroke
Cerebrovascular Circulation
Regional Blood Flow
Water
Isoflurane
Brain Ischemia
Stroke Volume
Ischemia

Keywords

  • Aquaporins
  • Cerebral edema
  • Focal cerebral ischemia
  • Infarct
  • Sex
  • Stroke

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Neurology

Cite this

Lack of sex-linked differences in cerebral edema and aquaporin-4 expression after experimental stroke. / Liu, Xiaoqin; Zhang, Wenri; Alkayed, Nabil; Froehner, Stanley C.; Adams, Marvin E.; Amiry-Moghaddam, Mahmood; Ottersen, Ole Petter; Hurn, Patricia D.; Bhardwaj, Anish.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 28, No. 12, 12.2008, p. 1898-1906.

Research output: Contribution to journalArticle

Liu, X, Zhang, W, Alkayed, N, Froehner, SC, Adams, ME, Amiry-Moghaddam, M, Ottersen, OP, Hurn, PD & Bhardwaj, A 2008, 'Lack of sex-linked differences in cerebral edema and aquaporin-4 expression after experimental stroke', Journal of Cerebral Blood Flow and Metabolism, vol. 28, no. 12, pp. 1898-1906. https://doi.org/10.1038/jcbfm.2008.83
Liu, Xiaoqin ; Zhang, Wenri ; Alkayed, Nabil ; Froehner, Stanley C. ; Adams, Marvin E. ; Amiry-Moghaddam, Mahmood ; Ottersen, Ole Petter ; Hurn, Patricia D. ; Bhardwaj, Anish. / Lack of sex-linked differences in cerebral edema and aquaporin-4 expression after experimental stroke. In: Journal of Cerebral Blood Flow and Metabolism. 2008 ; Vol. 28, No. 12. pp. 1898-1906.
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