Kinetics of spindle pole body separation in budding yeast

Jason A. Kahana, Bruce Schnapp, Pamela A. Silver

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

In the budding yeast Saccharomyces cerevisiae, the spindle pole body (SPB) serves as the microtubule-organizing center and is the functional analog of the centrosome of higher organisms. By expressing a fusion of a yeast SPB- associated protein to the Aequorea victoria green fluorescent protein, the movement of the SPBs in living yeast cells undergoing mitosis was observed by fluorescence microscopy. The ability to visualize SPBs in vivo has revealed previously unidentified mitotic events. During anaphase, the mitotic spindle has four sequential activities: alignment at the mother-daughter junction, fast elongation, translocation into the bud, and slow elongation. These results indicate that distinct forces act upon the spindle at different times during anaphase.

Original languageEnglish (US)
Pages (from-to)9707-9711
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number21
DOIs
StatePublished - Oct 10 1995
Externally publishedYes

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Spindle Pole Bodies
Anaphase
Saccharomycetales
Yeasts
Microtubule-Organizing Center
Centrosome
Spindle Apparatus
Fluorescence Microscopy
Mitosis
Saccharomyces cerevisiae
Proteins

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Kinetics of spindle pole body separation in budding yeast. / Kahana, Jason A.; Schnapp, Bruce; Silver, Pamela A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 21, 10.10.1995, p. 9707-9711.

Research output: Contribution to journalArticle

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