Isolation of mouse pancreatic alpha, beta, duct and acinar populations with cell surface markers

Craig Dorrell, Maria T. Grompe, Fong Cheng Pan, Yongping Zhong, Pamela S. Canaday, Leonard D. Shultz, Dale L. Greiner, Chris V. Wright, Philip Streeter, Markus Grompe

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Tools permitting the isolation of live pancreatic cell subsets for culture and/or molecular analysis are limited. To address this, we developed a collection of monoclonal antibodies with selective surface labeling of endocrine and exocrine pancreatic cell types. Cell type labeling specificity and cell surface reactivity were validated on mouse pancreatic sections and by gene expression analysis of cells isolated using FACS. Five antibodies which marked populations of particular interest were used to isolate and study viable populations of purified pancreatic ducts, acinar cells, and subsets of acinar cells from whole pancreatic tissue or of alpha or beta cells from isolated mouse islets. Gene expression analysis showed the presence of known endocrine markers in alpha and beta cell populations and revealed that TTR and DPPIV are primarily expressed in alpha cells whereas DGKB and GPM6A have a beta cell specific expression profile.

Original languageEnglish (US)
Pages (from-to)144-150
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume339
Issue number1-2
DOIs
StatePublished - Jun 6 2011

Fingerprint

Gene expression
Ducts
Labeling
Population
Monoclonal Antibodies
Cells
Tissue
Antibodies
Acinar Cells
Gene Expression
Pancreatic Ducts
Cell Culture Techniques

Keywords

  • Acinar
  • Alpha cell
  • Antibody
  • Beta cell
  • Duct
  • FACS

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Biochemistry

Cite this

Isolation of mouse pancreatic alpha, beta, duct and acinar populations with cell surface markers. / Dorrell, Craig; Grompe, Maria T.; Pan, Fong Cheng; Zhong, Yongping; Canaday, Pamela S.; Shultz, Leonard D.; Greiner, Dale L.; Wright, Chris V.; Streeter, Philip; Grompe, Markus.

In: Molecular and Cellular Endocrinology, Vol. 339, No. 1-2, 06.06.2011, p. 144-150.

Research output: Contribution to journalArticle

Dorrell, C, Grompe, MT, Pan, FC, Zhong, Y, Canaday, PS, Shultz, LD, Greiner, DL, Wright, CV, Streeter, P & Grompe, M 2011, 'Isolation of mouse pancreatic alpha, beta, duct and acinar populations with cell surface markers', Molecular and Cellular Endocrinology, vol. 339, no. 1-2, pp. 144-150. https://doi.org/10.1016/j.mce.2011.04.008
Dorrell, Craig ; Grompe, Maria T. ; Pan, Fong Cheng ; Zhong, Yongping ; Canaday, Pamela S. ; Shultz, Leonard D. ; Greiner, Dale L. ; Wright, Chris V. ; Streeter, Philip ; Grompe, Markus. / Isolation of mouse pancreatic alpha, beta, duct and acinar populations with cell surface markers. In: Molecular and Cellular Endocrinology. 2011 ; Vol. 339, No. 1-2. pp. 144-150.
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