Isolation of Drosophila CREB-B

A novel CRE-binding protein

T. Usui, S. M. Smolik, Richard Goodman

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

CREB is a DNA-binding protein that stimulates gene transcription upon activation of the cAMP signaling pathway. The mammalian CREB protein consists of an amino-terminal transcriptional activation domain and a carboxy-terminal DNA-binding domain comprised of a basic region and a leucine zipper. Recent studies have shown that the mammalian CREB is one of many transcription factors that can bind to the cAMP regulated enhancer (CRE) sequence. Consequently, a complete understanding of regulation through the CRE sequence requires the elucidation of how the various CRE-binding proteins interact with each other. To accomplish this goal, we have begun to characterize the family of CRE-binding proteins in a system that is amenable to genetic manipulations, Drosophila melanogaster. We have previously cloned a protein designated dCREB-A from a Drosophila embryonic cDNA library. Here, we describe an additional member of the Drosophila CREB gene family, isolated by screening a λgt11 library of adult Drosophila head cDNAs with a multimerized CRE sequence. This protein, dCREB-B, contains 285 amino acids and is remarkably similar within the basic/zipper region to the corresponding portion of mammalian CREB. In contrast, the dCREB-B and mammalian CREB zipper domains differ considerably from the dCREB-A zipper in both length and composition. However, the putative DNA binding domains for all three proteins are highly conserved. The activator region of dCREB-B is completely different from that of both mammalian CREB and dCREB-A. Northern blot analysis shows that multiple transcripts of the dCREB-B gene are expressed in embryonic and adult tissues and that these transcripts arise from both strands of the DNA. Chromosomal in situ hybridization shows that dCREB-B is located at position 17A-B on the X chromosome, the locus of several developmentally important genes. The structure of the dCREB-B gene is reminiscent of genes in the mammalian CREB family.

Original languageEnglish (US)
Pages (from-to)589-595
Number of pages7
JournalDNA and Cell Biology
Volume12
Issue number7
StatePublished - 1993

Fingerprint

Drosophila
Carrier Proteins
Genes
Transcriptional Activation
DNA
Leucine Zippers
Cyclic AMP Response Element-Binding Protein
DNA-Binding Proteins
X Chromosome
Drosophila melanogaster
Gene Library
Northern Blotting
Libraries
In Situ Hybridization
Proteins
Transcription Factors
Complementary DNA
Head
Amino Acids

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Isolation of Drosophila CREB-B : A novel CRE-binding protein. / Usui, T.; Smolik, S. M.; Goodman, Richard.

In: DNA and Cell Biology, Vol. 12, No. 7, 1993, p. 589-595.

Research output: Contribution to journalArticle

Usui, T. ; Smolik, S. M. ; Goodman, Richard. / Isolation of Drosophila CREB-B : A novel CRE-binding protein. In: DNA and Cell Biology. 1993 ; Vol. 12, No. 7. pp. 589-595.
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