Isolation of a Yeast Protein Kinase Gene by Screening with a Mammalian Protein Kinase cDNA

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The gene for a new Saccharomyces cerevisiae protein kinase was identified and isolated by hybridization to a cloned cDNA for the catalytic subunit of bovine cAMP-dependent protein kinase. This newly isolated yeast gene encodes a 680-amino-acid protein that shows strong sequence similarity to several serine/threonine protein kinases. The protein kinase catalytic domain is located at the carboxy-terminal portion of the protein. A large, amino-terminal domain shows no sequence similarity to any known protein kinase. It seems likely that the amino-terminal domain mediates the effects of an unknown regulator of the newly identified yeast protein kinase.

Original languageEnglish (US)
Pages (from-to)469-474
Number of pages6
Issue number7
StatePublished - Sep 1988


ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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