Isolation of a variant of subtilosin A with hemolytic activity

Tai Huang, Hao Geng, Venugopal R. Miyyapuram, Clarissa S. Sit, John C. Vederas, Michiko M. Nakano

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Bacillus subtilis produces an anionic bacteriocin called subtilosin A that possesses antibacterial activity against certain gram-positive bacteria. In this study, we uncovered a hemolytic mutant of B. subtilis that produces an altered form of subtilosin A. The mutant bacteriocin, named subtilosin A1, has a replacement of threonine at position 6 with isoleucine. In addition to the hemolytic activity, subtilosin A1 was found to exhibit enhanced antimicrobial activity against specific bacterial strains. The B. subtilis albB mutant that does not produce a putative immunity peptide was more sensitive to both subtilosin A and subtilosin A1. A spontaneous suppressor mutation of albB that restored resistance to subtilosin A and subtilosin A1 was obtained. The sbr (subtilosin resistance) mutation conferring the resistance is not linked to the sboA-alb locus. The sbr mutation does not increase the resistance of B. subtilis to other cell envelope-targeted antimicrobial agents, indicating that the mutation specifically confers the resistance to subtilosins. The findings suggest possible bioengineering approaches for obtaining anionic bacteriocins with enhanced and/or altered bactericidal activity. Furthermore, future identification of the subtilosin-resistant mutation could provide insights into the mechanism of subtilosin A activity.

Original languageEnglish (US)
Pages (from-to)5690-5696
Number of pages7
JournalJournal of bacteriology
Volume191
Issue number18
DOIs
StatePublished - Sep 2009

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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