Isolation of a variant of subtilosin A with hemolytic activity

Tai Huang, Hao Geng, Venugopal R. Miyyapuram, Clarissa S. Sit, John C. Vederas, Michiko Nakano

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Bacillus subtilis produces an anionic bacteriocin called subtilosin A that possesses antibacterial activity against certain gram-positive bacteria. In this study, we uncovered a hemolytic mutant of B. subtilis that produces an altered form of subtilosin A. The mutant bacteriocin, named subtilosin A1, has a replacement of threonine at position 6 with isoleucine. In addition to the hemolytic activity, subtilosin A1 was found to exhibit enhanced antimicrobial activity against specific bacterial strains. The B. subtilis albB mutant that does not produce a putative immunity peptide was more sensitive to both subtilosin A and subtilosin A1. A spontaneous suppressor mutation of albB that restored resistance to subtilosin A and subtilosin A1 was obtained. The sbr (subtilosin resistance) mutation conferring the resistance is not linked to the sboA-alb locus. The sbr mutation does not increase the resistance of B. subtilis to other cell envelope-targeted antimicrobial agents, indicating that the mutation specifically confers the resistance to subtilosins. The findings suggest possible bioengineering approaches for obtaining anionic bacteriocins with enhanced and/or altered bactericidal activity. Furthermore, future identification of the subtilosin-resistant mutation could provide insights into the mechanism of subtilosin A activity.

Original languageEnglish (US)
Pages (from-to)5690-5696
Number of pages7
JournalJournal of Bacteriology
Volume191
Issue number18
DOIs
StatePublished - Sep 2009

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Bacillus subtilis
Bacteriocins
Mutation
Bacillus subtilis sboA protein
Genetic Suppression
Bioengineering
Isoleucine
Gram-Positive Bacteria
Threonine
Anti-Infective Agents
Immunity
Peptides

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Huang, T., Geng, H., Miyyapuram, V. R., Sit, C. S., Vederas, J. C., & Nakano, M. (2009). Isolation of a variant of subtilosin A with hemolytic activity. Journal of Bacteriology, 191(18), 5690-5696. https://doi.org/10.1128/JB.00541-09

Isolation of a variant of subtilosin A with hemolytic activity. / Huang, Tai; Geng, Hao; Miyyapuram, Venugopal R.; Sit, Clarissa S.; Vederas, John C.; Nakano, Michiko.

In: Journal of Bacteriology, Vol. 191, No. 18, 09.2009, p. 5690-5696.

Research output: Contribution to journalArticle

Huang, T, Geng, H, Miyyapuram, VR, Sit, CS, Vederas, JC & Nakano, M 2009, 'Isolation of a variant of subtilosin A with hemolytic activity', Journal of Bacteriology, vol. 191, no. 18, pp. 5690-5696. https://doi.org/10.1128/JB.00541-09
Huang, Tai ; Geng, Hao ; Miyyapuram, Venugopal R. ; Sit, Clarissa S. ; Vederas, John C. ; Nakano, Michiko. / Isolation of a variant of subtilosin A with hemolytic activity. In: Journal of Bacteriology. 2009 ; Vol. 191, No. 18. pp. 5690-5696.
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