Isolation of a human allo-peptide presented by HLA-B51 molecules

H. Tomiyama, Y. Takamiya, A. B. Hill, V. Cerundolo, A. Kelly, K. Egawa, J. Trowsdale, M. Takiguchi

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Recent studies have demonstrated directly that alloreactive mouse CTL recognize peptides presented by MHC class I molecules. However, there is no direct evidence that human alloreactive CTL recognize peptides presented by HLA class I molecules. We have isolated an HLA-B51 alloreactive CTL clone, 283, that did not kill the TAP defective cell lines T2 and.174, whereas it killed the TAP-positive cell line T1 and.174 cells transfected with TAP genes. These findings suggested that this clone recognizes a TAP-dependent allo-peptide. We attempted to isolate the human allo-peptide recognized by the 283 clone from HLA-B51 molecules. A naturally occurring HLA-B(*)5101 binding peptide isolated from T1 cells was recognized by the 283 clone. The peptide was also isolated from HLA-B(*)5101 molecules purified from C1R-B(*)5101 cells. In the present study, we directly demonstrated that a human alloreactive CTL clone recognizes peptide presented by HLA class I molecules.

Original languageEnglish (US)
Pages (from-to)905-909
Number of pages5
JournalInternational Immunology
Volume7
Issue number6
DOIs
StatePublished - 1995

Keywords

  • Allo-peptide
  • Allorecognition
  • Class I
  • HLA
  • Presentation
  • Tap

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Tomiyama, H., Takamiya, Y., Hill, A. B., Cerundolo, V., Kelly, A., Egawa, K., Trowsdale, J., & Takiguchi, M. (1995). Isolation of a human allo-peptide presented by HLA-B51 molecules. International Immunology, 7(6), 905-909. https://doi.org/10.1093/intimm/7.6.905