Isolation of a human allo-peptide presented by HLA-B51 molecules

H. Tomiyama, Y. Takamiya, Ann Hill, V. Cerundolo, A. Kelly, K. Egawa, J. Trowsdale, M. Takiguchi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Recent studies have demonstrated directly that alloreactive mouse CTL recognize peptides presented by MHC class I molecules. However, there is no direct evidence that human alloreactive CTL recognize peptides presented by HLA class I molecules. We have isolated an HLA-B51 alloreactive CTL clone, 283, that did not kill the TAP defective cell lines T2 and.174, whereas it killed the TAP-positive cell line T1 and.174 cells transfected with TAP genes. These findings suggested that this clone recognizes a TAP-dependent allo-peptide. We attempted to isolate the human allo-peptide recognized by the 283 clone from HLA-B51 molecules. A naturally occurring HLA-B(*)5101 binding peptide isolated from T1 cells was recognized by the 283 clone. The peptide was also isolated from HLA-B(*)5101 molecules purified from C1R-B(*)5101 cells. In the present study, we directly demonstrated that a human alloreactive CTL clone recognizes peptide presented by HLA class I molecules.

Original languageEnglish (US)
Pages (from-to)905-909
Number of pages5
JournalInternational Immunology
Volume7
Issue number6
StatePublished - 1995
Externally publishedYes

Fingerprint

HLA-B51 Antigen
Peptides
Clone Cells
HLA-B Antigens
Cell Line

Keywords

  • Allo-peptide
  • Allorecognition
  • Class I
  • HLA
  • Presentation
  • Tap

ASJC Scopus subject areas

  • Immunology

Cite this

Tomiyama, H., Takamiya, Y., Hill, A., Cerundolo, V., Kelly, A., Egawa, K., ... Takiguchi, M. (1995). Isolation of a human allo-peptide presented by HLA-B51 molecules. International Immunology, 7(6), 905-909.

Isolation of a human allo-peptide presented by HLA-B51 molecules. / Tomiyama, H.; Takamiya, Y.; Hill, Ann; Cerundolo, V.; Kelly, A.; Egawa, K.; Trowsdale, J.; Takiguchi, M.

In: International Immunology, Vol. 7, No. 6, 1995, p. 905-909.

Research output: Contribution to journalArticle

Tomiyama, H, Takamiya, Y, Hill, A, Cerundolo, V, Kelly, A, Egawa, K, Trowsdale, J & Takiguchi, M 1995, 'Isolation of a human allo-peptide presented by HLA-B51 molecules', International Immunology, vol. 7, no. 6, pp. 905-909.
Tomiyama H, Takamiya Y, Hill A, Cerundolo V, Kelly A, Egawa K et al. Isolation of a human allo-peptide presented by HLA-B51 molecules. International Immunology. 1995;7(6):905-909.
Tomiyama, H. ; Takamiya, Y. ; Hill, Ann ; Cerundolo, V. ; Kelly, A. ; Egawa, K. ; Trowsdale, J. ; Takiguchi, M. / Isolation of a human allo-peptide presented by HLA-B51 molecules. In: International Immunology. 1995 ; Vol. 7, No. 6. pp. 905-909.
@article{10b6c682fbd94bb689fd6e6731575698,
title = "Isolation of a human allo-peptide presented by HLA-B51 molecules",
abstract = "Recent studies have demonstrated directly that alloreactive mouse CTL recognize peptides presented by MHC class I molecules. However, there is no direct evidence that human alloreactive CTL recognize peptides presented by HLA class I molecules. We have isolated an HLA-B51 alloreactive CTL clone, 283, that did not kill the TAP defective cell lines T2 and.174, whereas it killed the TAP-positive cell line T1 and.174 cells transfected with TAP genes. These findings suggested that this clone recognizes a TAP-dependent allo-peptide. We attempted to isolate the human allo-peptide recognized by the 283 clone from HLA-B51 molecules. A naturally occurring HLA-B(*)5101 binding peptide isolated from T1 cells was recognized by the 283 clone. The peptide was also isolated from HLA-B(*)5101 molecules purified from C1R-B(*)5101 cells. In the present study, we directly demonstrated that a human alloreactive CTL clone recognizes peptide presented by HLA class I molecules.",
keywords = "Allo-peptide, Allorecognition, Class I, HLA, Presentation, Tap",
author = "H. Tomiyama and Y. Takamiya and Ann Hill and V. Cerundolo and A. Kelly and K. Egawa and J. Trowsdale and M. Takiguchi",
year = "1995",
language = "English (US)",
volume = "7",
pages = "905--909",
journal = "International Immunology",
issn = "0953-8178",
publisher = "Oxford University Press",
number = "6",

}

TY - JOUR

T1 - Isolation of a human allo-peptide presented by HLA-B51 molecules

AU - Tomiyama, H.

AU - Takamiya, Y.

AU - Hill, Ann

AU - Cerundolo, V.

AU - Kelly, A.

AU - Egawa, K.

AU - Trowsdale, J.

AU - Takiguchi, M.

PY - 1995

Y1 - 1995

N2 - Recent studies have demonstrated directly that alloreactive mouse CTL recognize peptides presented by MHC class I molecules. However, there is no direct evidence that human alloreactive CTL recognize peptides presented by HLA class I molecules. We have isolated an HLA-B51 alloreactive CTL clone, 283, that did not kill the TAP defective cell lines T2 and.174, whereas it killed the TAP-positive cell line T1 and.174 cells transfected with TAP genes. These findings suggested that this clone recognizes a TAP-dependent allo-peptide. We attempted to isolate the human allo-peptide recognized by the 283 clone from HLA-B51 molecules. A naturally occurring HLA-B(*)5101 binding peptide isolated from T1 cells was recognized by the 283 clone. The peptide was also isolated from HLA-B(*)5101 molecules purified from C1R-B(*)5101 cells. In the present study, we directly demonstrated that a human alloreactive CTL clone recognizes peptide presented by HLA class I molecules.

AB - Recent studies have demonstrated directly that alloreactive mouse CTL recognize peptides presented by MHC class I molecules. However, there is no direct evidence that human alloreactive CTL recognize peptides presented by HLA class I molecules. We have isolated an HLA-B51 alloreactive CTL clone, 283, that did not kill the TAP defective cell lines T2 and.174, whereas it killed the TAP-positive cell line T1 and.174 cells transfected with TAP genes. These findings suggested that this clone recognizes a TAP-dependent allo-peptide. We attempted to isolate the human allo-peptide recognized by the 283 clone from HLA-B51 molecules. A naturally occurring HLA-B(*)5101 binding peptide isolated from T1 cells was recognized by the 283 clone. The peptide was also isolated from HLA-B(*)5101 molecules purified from C1R-B(*)5101 cells. In the present study, we directly demonstrated that a human alloreactive CTL clone recognizes peptide presented by HLA class I molecules.

KW - Allo-peptide

KW - Allorecognition

KW - Class I

KW - HLA

KW - Presentation

KW - Tap

UR - http://www.scopus.com/inward/record.url?scp=0029008165&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029008165&partnerID=8YFLogxK

M3 - Article

VL - 7

SP - 905

EP - 909

JO - International Immunology

JF - International Immunology

SN - 0953-8178

IS - 6

ER -