Isolation and detection of cancer related DNA nanoparticulate biomarkers directly from CLL patient blood "rapid sample to sequence diagnostics"

Michael (Mike) Heller, Jennifer Marciniak, Avery Sonnenberg

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have now demonstrated a dielectrophoretic (DEP) based approach that allows a full range of cfc-DNA biomarkers from chronic lymphocytic leukemia (CLL) patients to be isolated directly from a small volume of unprocessed whole blood. Using a DEP microarray device, cfc-DNA from 50 uls of patient blood is separated and concentrated into DEP high-field regions in about 10-15 minutes. Blood cells, serum proteins and low molecular biomolecules are quickly removed by a fluidic wash. The cfc-DNA concentrated on the DEP microelectrodes is first analyzed by fluorescence, and then eluted with a fluidic wash for subsequent PCR and sequencing analysis. In a majority of CLL samples, fluorescent stained cfc-DNA was clearly observed on the DEP microelectrodes, while little or no fluorescence was observed for the normal blood control samples. Eluted cfc-DNA (equivalent to 2ul-5ul of original blood sample) was then PCR amplified using VHL specific primers, which verified the CLL patient type for 29 of 35 CLL samples. The CLL patients amplified DNA was then sequenced and correlated with previous patient specific sequencing results from DNA originally isolated from white blood cells.

Original languageEnglish (US)
Title of host publicationTechnical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
Pages392-395
Number of pages4
StatePublished - Aug 9 2013
Externally publishedYes
EventNanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 - Washington, DC, United States
Duration: May 12 2013May 16 2013

Publication series

NameTechnical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
Volume3

Conference

ConferenceNanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013
CountryUnited States
CityWashington, DC
Period5/12/135/16/13

Fingerprint

B-Cell Chronic Lymphocytic Leukemia
Biomarkers
DNA
Neoplasms
Microelectrodes
Fluorescence
Polymerase Chain Reaction
DNA Sequence Analysis
Blood Proteins
Blood Cells
Leukocytes
Equipment and Supplies
Serum

Keywords

  • Blood
  • Cancer
  • Dielectrophoresis
  • DNA biomarkers

ASJC Scopus subject areas

  • Biotechnology

Cite this

Heller, M. M., Marciniak, J., & Sonnenberg, A. (2013). Isolation and detection of cancer related DNA nanoparticulate biomarkers directly from CLL patient blood "rapid sample to sequence diagnostics". In Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013 (pp. 392-395). (Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013; Vol. 3).

Isolation and detection of cancer related DNA nanoparticulate biomarkers directly from CLL patient blood "rapid sample to sequence diagnostics". / Heller, Michael (Mike); Marciniak, Jennifer; Sonnenberg, Avery.

Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. 2013. p. 392-395 (Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013; Vol. 3).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Heller, MM, Marciniak, J & Sonnenberg, A 2013, Isolation and detection of cancer related DNA nanoparticulate biomarkers directly from CLL patient blood "rapid sample to sequence diagnostics". in Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013, vol. 3, pp. 392-395, Nanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy - 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013, Washington, DC, United States, 5/12/13.
Heller MM, Marciniak J, Sonnenberg A. Isolation and detection of cancer related DNA nanoparticulate biomarkers directly from CLL patient blood "rapid sample to sequence diagnostics". In Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. 2013. p. 392-395. (Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013).
Heller, Michael (Mike) ; Marciniak, Jennifer ; Sonnenberg, Avery. / Isolation and detection of cancer related DNA nanoparticulate biomarkers directly from CLL patient blood "rapid sample to sequence diagnostics". Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013. 2013. pp. 392-395 (Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2013).
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