The present study investigates the possible relationship between human β-cell secretory capacity and polymorphism in the 5'-flanking region of the human insulin gene. The glucose potentiation slope was measured in normal and non-insulin-dependent diabetic subjects (NIDDM). This slope, as reported previously, is an index of the ability of hyperglycemia to potentiate the insulin response the arginine and as such is a measure of β-cell responsiveness to glucose. Restriction enzyme analysis using a human insulin gene probe was performed on leukocyte DNA isolated from the same individuals. We conclude that a 1.6 kb polymorphism in the 5'-flanking region of the human insulin gene in both normal and NIDDM subjects has no association with insulin secretory responss as defined here by the glucose potentiation slope.
ASJC Scopus subject areas
- Internal Medicine
- Endocrinology, Diabetes and Metabolism