Ischemic memory imaging in nonhuman primates with echocardiographic molecular imaging of selectin expression

Brian P. Davidson, Scott Chadderdon, J. Todd Belcik, Saurabh Gupta, Jonathan Lindner

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    Background Selectins are adhesion molecules that are expressed by the vascular endothelium upon activation and may be an imaging target for detecting myocardial ischemia long after resolution. The aim of this study was to test the hypothesis that molecular imaging of selectins with myocardial contrast echocardiographic (MCE) molecular imaging could be used to detect recent brief ischemia in closed-chest nonhuman primates. Methods Myocardial ischemia was produced in anesthetized adult rhesus macaques (n = 6) by percutaneous balloon catheter occlusion of the left anterior descending or circumflex coronary artery for 5 to 10 min. Three separate macaques served as nonischemic controls. MCE perfusion imaging was performed during coronary occlusion to measure risk area and at 100 to 110 min to exclude infarction. MCE molecular imaging was performed at 30 and 90 min after reperfusion using a lipid microbubble bearing dimeric recombinant human P-selectin glycoprotein ligand-1 (MB-YSPSL). Collection of blood for safety data, electrocardiography, and echocardiography were performed at baseline and before and 10 min after each MB-YSPSL injection. Results Vital signs, oxygen saturation, electrocardiographic results, ventricular systolic function, pulmonary vascular resistance, and serum safety markers were unchanged by intravenous injection of MB-YSPSL. On echocardiography, left ventricular dysfunction in the risk area had resolved by 30 min, and there was no evidence of infarction on MCE perfusion imaging. On selectin-targeted MCE molecular imaging, signal enhancement was greater (P

    Original languageEnglish (US)
    JournalJournal of the American Society of Echocardiography
    Volume27
    Issue number7
    DOIs
    StatePublished - 2014

    Fingerprint

    Selectins
    Molecular Imaging
    Primates
    Perfusion Imaging
    Myocardial Ischemia
    Echocardiography
    Blood Safety
    Balloon Occlusion
    Microbubbles
    Ventricular Function
    Vital Signs
    Coronary Occlusion
    Vascular Endothelium
    Macaca
    Left Ventricular Dysfunction
    Macaca mulatta
    Intravenous Injections
    Vascular Resistance
    Infarction
    Reperfusion

    Keywords

    • Contrast echocardiography
    • Ischemia
    • Molecular imaging
    • Selectins

    ASJC Scopus subject areas

    • Radiology Nuclear Medicine and imaging
    • Cardiology and Cardiovascular Medicine
    • Medicine(all)

    Cite this

    Ischemic memory imaging in nonhuman primates with echocardiographic molecular imaging of selectin expression. / Davidson, Brian P.; Chadderdon, Scott; Belcik, J. Todd; Gupta, Saurabh; Lindner, Jonathan.

    In: Journal of the American Society of Echocardiography, Vol. 27, No. 7, 2014.

    Research output: Contribution to journalArticle

    @article{1a4a796e207d422db68427d7b89d24d9,
    title = "Ischemic memory imaging in nonhuman primates with echocardiographic molecular imaging of selectin expression",
    abstract = "Background Selectins are adhesion molecules that are expressed by the vascular endothelium upon activation and may be an imaging target for detecting myocardial ischemia long after resolution. The aim of this study was to test the hypothesis that molecular imaging of selectins with myocardial contrast echocardiographic (MCE) molecular imaging could be used to detect recent brief ischemia in closed-chest nonhuman primates. Methods Myocardial ischemia was produced in anesthetized adult rhesus macaques (n = 6) by percutaneous balloon catheter occlusion of the left anterior descending or circumflex coronary artery for 5 to 10 min. Three separate macaques served as nonischemic controls. MCE perfusion imaging was performed during coronary occlusion to measure risk area and at 100 to 110 min to exclude infarction. MCE molecular imaging was performed at 30 and 90 min after reperfusion using a lipid microbubble bearing dimeric recombinant human P-selectin glycoprotein ligand-1 (MB-YSPSL). Collection of blood for safety data, electrocardiography, and echocardiography were performed at baseline and before and 10 min after each MB-YSPSL injection. Results Vital signs, oxygen saturation, electrocardiographic results, ventricular systolic function, pulmonary vascular resistance, and serum safety markers were unchanged by intravenous injection of MB-YSPSL. On echocardiography, left ventricular dysfunction in the risk area had resolved by 30 min, and there was no evidence of infarction on MCE perfusion imaging. On selectin-targeted MCE molecular imaging, signal enhancement was greater (P",
    keywords = "Contrast echocardiography, Ischemia, Molecular imaging, Selectins",
    author = "Davidson, {Brian P.} and Scott Chadderdon and Belcik, {J. Todd} and Saurabh Gupta and Jonathan Lindner",
    year = "2014",
    doi = "10.1016/j.echo.2014.03.013",
    language = "English (US)",
    volume = "27",
    journal = "Journal of the American Society of Echocardiography",
    issn = "0894-7317",
    publisher = "Mosby Inc.",
    number = "7",

    }

    TY - JOUR

    T1 - Ischemic memory imaging in nonhuman primates with echocardiographic molecular imaging of selectin expression

    AU - Davidson, Brian P.

    AU - Chadderdon, Scott

    AU - Belcik, J. Todd

    AU - Gupta, Saurabh

    AU - Lindner, Jonathan

    PY - 2014

    Y1 - 2014

    N2 - Background Selectins are adhesion molecules that are expressed by the vascular endothelium upon activation and may be an imaging target for detecting myocardial ischemia long after resolution. The aim of this study was to test the hypothesis that molecular imaging of selectins with myocardial contrast echocardiographic (MCE) molecular imaging could be used to detect recent brief ischemia in closed-chest nonhuman primates. Methods Myocardial ischemia was produced in anesthetized adult rhesus macaques (n = 6) by percutaneous balloon catheter occlusion of the left anterior descending or circumflex coronary artery for 5 to 10 min. Three separate macaques served as nonischemic controls. MCE perfusion imaging was performed during coronary occlusion to measure risk area and at 100 to 110 min to exclude infarction. MCE molecular imaging was performed at 30 and 90 min after reperfusion using a lipid microbubble bearing dimeric recombinant human P-selectin glycoprotein ligand-1 (MB-YSPSL). Collection of blood for safety data, electrocardiography, and echocardiography were performed at baseline and before and 10 min after each MB-YSPSL injection. Results Vital signs, oxygen saturation, electrocardiographic results, ventricular systolic function, pulmonary vascular resistance, and serum safety markers were unchanged by intravenous injection of MB-YSPSL. On echocardiography, left ventricular dysfunction in the risk area had resolved by 30 min, and there was no evidence of infarction on MCE perfusion imaging. On selectin-targeted MCE molecular imaging, signal enhancement was greater (P

    AB - Background Selectins are adhesion molecules that are expressed by the vascular endothelium upon activation and may be an imaging target for detecting myocardial ischemia long after resolution. The aim of this study was to test the hypothesis that molecular imaging of selectins with myocardial contrast echocardiographic (MCE) molecular imaging could be used to detect recent brief ischemia in closed-chest nonhuman primates. Methods Myocardial ischemia was produced in anesthetized adult rhesus macaques (n = 6) by percutaneous balloon catheter occlusion of the left anterior descending or circumflex coronary artery for 5 to 10 min. Three separate macaques served as nonischemic controls. MCE perfusion imaging was performed during coronary occlusion to measure risk area and at 100 to 110 min to exclude infarction. MCE molecular imaging was performed at 30 and 90 min after reperfusion using a lipid microbubble bearing dimeric recombinant human P-selectin glycoprotein ligand-1 (MB-YSPSL). Collection of blood for safety data, electrocardiography, and echocardiography were performed at baseline and before and 10 min after each MB-YSPSL injection. Results Vital signs, oxygen saturation, electrocardiographic results, ventricular systolic function, pulmonary vascular resistance, and serum safety markers were unchanged by intravenous injection of MB-YSPSL. On echocardiography, left ventricular dysfunction in the risk area had resolved by 30 min, and there was no evidence of infarction on MCE perfusion imaging. On selectin-targeted MCE molecular imaging, signal enhancement was greater (P

    KW - Contrast echocardiography

    KW - Ischemia

    KW - Molecular imaging

    KW - Selectins

    UR - http://www.scopus.com/inward/record.url?scp=84903157265&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84903157265&partnerID=8YFLogxK

    U2 - 10.1016/j.echo.2014.03.013

    DO - 10.1016/j.echo.2014.03.013

    M3 - Article

    C2 - 24774222

    AN - SCOPUS:84903157265

    VL - 27

    JO - Journal of the American Society of Echocardiography

    JF - Journal of the American Society of Echocardiography

    SN - 0894-7317

    IS - 7

    ER -