Is the omega-3 index a valid marker of intestinal membrane phospholipid EPA+DHA content?

Eric A. Gurzell, Jason A. Wiesinger, Christina Morkam, Sophia Hemmrich, William Harris, Jenifer I. Fenton

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Despite numerous studies investigating n-3 long chain polyunsaturated fatty acid (LCPUFA) supplementation and inflammatory bowel diseases (IBD), the extent to which dietary n-3 LCPUFAs incorporate in gastrointestinal (GI) tissues and correlate with red blood cell (RBC) n-3 LCPUFA content is unknown. In this study, mice were fed three diets with increasing percent of energy (%en) derived from eicosapentaenoic acid (EPA)+docosahexaenoic acid (DHA). Dietary levels reflected recommended intakes of fish/fish oil by the American Heart Association. We analyzed the FA composition of phospholipids extracted from RBCs, plasma, and GI tissues. We observed that the 0.1%en EPA+DHA diet was sufficient to significantly increase the omega-3 index (RBC EPA+DHA) after 5 week feeding. The baseline EPA levels were 0.2-0.6% across all tissues increasing to 1.6-4.3% in the highest EPA+DHA diet; these changes resulted in absolute increases of 1.4-3.9% EPA across tissues. The baseline DHA levels were 2.2-5.9% across all tissues increasing to 5.8-10.5% in the highest EPA+DHA diet; these changes resulted in absolute increases of 3.2-5.7% DHA across tissues. These increases in EPA and DHA across all tissues resulted in strong (r>0.91) and significant (P

Original languageEnglish (US)
Pages (from-to)87-96
Number of pages10
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume91
Issue number3
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Eicosapentaenoic Acid
Docosahexaenoic Acids
Phospholipids
Membranes
Tissue
Nutrition
Diet
Unsaturated Fatty Acids
Blood
Erythrocytes
Fish Oils
Inflammatory Bowel Diseases
Fish
Fishes
Cells
Plasmas
Chemical analysis

Keywords

  • Biomarker
  • Colon
  • Fatty acids
  • Intestine
  • Omega-3
  • Red blood cell

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Medicine(all)

Cite this

Is the omega-3 index a valid marker of intestinal membrane phospholipid EPA+DHA content? / Gurzell, Eric A.; Wiesinger, Jason A.; Morkam, Christina; Hemmrich, Sophia; Harris, William; Fenton, Jenifer I.

In: Prostaglandins Leukotrienes and Essential Fatty Acids, Vol. 91, No. 3, 2014, p. 87-96.

Research output: Contribution to journalArticle

Gurzell, Eric A. ; Wiesinger, Jason A. ; Morkam, Christina ; Hemmrich, Sophia ; Harris, William ; Fenton, Jenifer I. / Is the omega-3 index a valid marker of intestinal membrane phospholipid EPA+DHA content?. In: Prostaglandins Leukotrienes and Essential Fatty Acids. 2014 ; Vol. 91, No. 3. pp. 87-96.
@article{1a2d69b6327041419f6d8cd0c514b3c9,
title = "Is the omega-3 index a valid marker of intestinal membrane phospholipid EPA+DHA content?",
abstract = "Despite numerous studies investigating n-3 long chain polyunsaturated fatty acid (LCPUFA) supplementation and inflammatory bowel diseases (IBD), the extent to which dietary n-3 LCPUFAs incorporate in gastrointestinal (GI) tissues and correlate with red blood cell (RBC) n-3 LCPUFA content is unknown. In this study, mice were fed three diets with increasing percent of energy ({\%}en) derived from eicosapentaenoic acid (EPA)+docosahexaenoic acid (DHA). Dietary levels reflected recommended intakes of fish/fish oil by the American Heart Association. We analyzed the FA composition of phospholipids extracted from RBCs, plasma, and GI tissues. We observed that the 0.1{\%}en EPA+DHA diet was sufficient to significantly increase the omega-3 index (RBC EPA+DHA) after 5 week feeding. The baseline EPA levels were 0.2-0.6{\%} across all tissues increasing to 1.6-4.3{\%} in the highest EPA+DHA diet; these changes resulted in absolute increases of 1.4-3.9{\%} EPA across tissues. The baseline DHA levels were 2.2-5.9{\%} across all tissues increasing to 5.8-10.5{\%} in the highest EPA+DHA diet; these changes resulted in absolute increases of 3.2-5.7{\%} DHA across tissues. These increases in EPA and DHA across all tissues resulted in strong (r>0.91) and significant (P",
keywords = "Biomarker, Colon, Fatty acids, Intestine, Omega-3, Red blood cell",
author = "Gurzell, {Eric A.} and Wiesinger, {Jason A.} and Christina Morkam and Sophia Hemmrich and William Harris and Fenton, {Jenifer I.}",
year = "2014",
doi = "10.1016/j.plefa.2014.04.001",
language = "English (US)",
volume = "91",
pages = "87--96",
journal = "Prostaglandins Leukotrienes and Essential Fatty Acids",
issn = "0952-3278",
publisher = "Churchill Livingstone",
number = "3",

}

TY - JOUR

T1 - Is the omega-3 index a valid marker of intestinal membrane phospholipid EPA+DHA content?

AU - Gurzell, Eric A.

AU - Wiesinger, Jason A.

AU - Morkam, Christina

AU - Hemmrich, Sophia

AU - Harris, William

AU - Fenton, Jenifer I.

PY - 2014

Y1 - 2014

N2 - Despite numerous studies investigating n-3 long chain polyunsaturated fatty acid (LCPUFA) supplementation and inflammatory bowel diseases (IBD), the extent to which dietary n-3 LCPUFAs incorporate in gastrointestinal (GI) tissues and correlate with red blood cell (RBC) n-3 LCPUFA content is unknown. In this study, mice were fed three diets with increasing percent of energy (%en) derived from eicosapentaenoic acid (EPA)+docosahexaenoic acid (DHA). Dietary levels reflected recommended intakes of fish/fish oil by the American Heart Association. We analyzed the FA composition of phospholipids extracted from RBCs, plasma, and GI tissues. We observed that the 0.1%en EPA+DHA diet was sufficient to significantly increase the omega-3 index (RBC EPA+DHA) after 5 week feeding. The baseline EPA levels were 0.2-0.6% across all tissues increasing to 1.6-4.3% in the highest EPA+DHA diet; these changes resulted in absolute increases of 1.4-3.9% EPA across tissues. The baseline DHA levels were 2.2-5.9% across all tissues increasing to 5.8-10.5% in the highest EPA+DHA diet; these changes resulted in absolute increases of 3.2-5.7% DHA across tissues. These increases in EPA and DHA across all tissues resulted in strong (r>0.91) and significant (P

AB - Despite numerous studies investigating n-3 long chain polyunsaturated fatty acid (LCPUFA) supplementation and inflammatory bowel diseases (IBD), the extent to which dietary n-3 LCPUFAs incorporate in gastrointestinal (GI) tissues and correlate with red blood cell (RBC) n-3 LCPUFA content is unknown. In this study, mice were fed three diets with increasing percent of energy (%en) derived from eicosapentaenoic acid (EPA)+docosahexaenoic acid (DHA). Dietary levels reflected recommended intakes of fish/fish oil by the American Heart Association. We analyzed the FA composition of phospholipids extracted from RBCs, plasma, and GI tissues. We observed that the 0.1%en EPA+DHA diet was sufficient to significantly increase the omega-3 index (RBC EPA+DHA) after 5 week feeding. The baseline EPA levels were 0.2-0.6% across all tissues increasing to 1.6-4.3% in the highest EPA+DHA diet; these changes resulted in absolute increases of 1.4-3.9% EPA across tissues. The baseline DHA levels were 2.2-5.9% across all tissues increasing to 5.8-10.5% in the highest EPA+DHA diet; these changes resulted in absolute increases of 3.2-5.7% DHA across tissues. These increases in EPA and DHA across all tissues resulted in strong (r>0.91) and significant (P

KW - Biomarker

KW - Colon

KW - Fatty acids

KW - Intestine

KW - Omega-3

KW - Red blood cell

UR - http://www.scopus.com/inward/record.url?scp=84905592450&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84905592450&partnerID=8YFLogxK

U2 - 10.1016/j.plefa.2014.04.001

DO - 10.1016/j.plefa.2014.04.001

M3 - Article

C2 - 24913088

AN - SCOPUS:84905592450

VL - 91

SP - 87

EP - 96

JO - Prostaglandins Leukotrienes and Essential Fatty Acids

JF - Prostaglandins Leukotrienes and Essential Fatty Acids

SN - 0952-3278

IS - 3

ER -