Irreversible inhibition by acetaldehyde of cholecystokinin-induced amylase secretion from isolated rat pancreatic acini

Hariharan Sankaran, Mark B. Lewin, Anny Wong, Clifford W. Deveney, Michael F. Wendland, Richard M. Leimgruber, Michael C. Geokas

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Acetaldehyde inhibited both amylase secretion induced by maximal concentrations (300 pM) of cholecystokinin octapeptide and the binding of radioiodinated cholecystokinin to receptors on isolated rat pancreatic acini. This inhibition was concentration dependent (10 mM to 1M for amylase secretion and 100 mM to 1M for binding). However, a correlation between the two inhibitory effects could not be obtained. Furthermore, the inhibitory effects were not reversible. Acetaldehyde did not alter the basal amylase secretion between 6 and 45 mM concentrations. However, 60, 100 and 300 mM acetaldehyde significantly decreased basal amylase secretion; no significant change in amylase secretion was observed at 600 mM and 1M. Higher concentrations of acetaldehyde produced a 2- to 10-fold increase in basal amylase secretion. 51Cr release from prelabeled acini revealed no significant cell membrane damage between 10 and 600 mM acetaldehyde. These data suggest that acetaldehyde inhibition of cholecystokinin-induced amylase secretion is intracellularly mediated.

Original languageEnglish (US)
Pages (from-to)2859-2863
Number of pages5
JournalBiochemical Pharmacology
Issue number16
StatePublished - Aug 15 1985


ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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