Ionic Composition of Microdialysis Perfusing Solution Alters the Pharmacological Responsiveness and Basal Outflow of Striatal Dopamine

Bita Moghaddam, Benjamin S. Bunney

Research output: Contribution to journalArticle

262 Citations (Scopus)

Abstract

Abstract: While using the technique of in vivo microdialysis, we have assessed the effect of the ionic composition of the perfusing solution on extracellular dopamine levels during resting conditions and following a pharmacological manipulation. Our results indicate that perfusion with solutions containing the ionic composition of commercially available Ringer's solution, which mimic the ionic composition of plasma as opposed to brain extracellular fluid, alters the turnover rate and basal release of dopamine. Moreover, perfusion with solutions containing higher calcium levels, i.e., 3.4 mM, than the amount we have determined to be present in the extracellular fluid of striatum (1.2 mM) alters the pharmacological responsiveness of the nigrostriatal dopamine system to synthesis inhibition.

Original languageEnglish (US)
Pages (from-to)652-654
Number of pages3
JournalJournal of Neurochemistry
Volume53
Issue number2
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Corpus Striatum
Microdialysis
Dopamine
Extracellular Fluid
Pharmacology
Perfusion
Chemical analysis
Fluids
Brain
Calcium
Plasmas

Keywords

  • Calcium
  • Dopamine
  • In vivo release
  • Ion‐selective microelectrodes
  • Microanalysis
  • α‐Methyl‐p‐tyrosine

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

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