Ionic Composition of Microdialysis Perfusing Solution Alters the Pharmacological Responsiveness and Basal Outflow of Striatal Dopamine

Bita Moghaddam, Benjamin S. Bunney

Research output: Contribution to journalArticle

262 Scopus citations

Abstract

Abstract: While using the technique of in vivo microdialysis, we have assessed the effect of the ionic composition of the perfusing solution on extracellular dopamine levels during resting conditions and following a pharmacological manipulation. Our results indicate that perfusion with solutions containing the ionic composition of commercially available Ringer's solution, which mimic the ionic composition of plasma as opposed to brain extracellular fluid, alters the turnover rate and basal release of dopamine. Moreover, perfusion with solutions containing higher calcium levels, i.e., 3.4 mM, than the amount we have determined to be present in the extracellular fluid of striatum (1.2 mM) alters the pharmacological responsiveness of the nigrostriatal dopamine system to synthesis inhibition.

Original languageEnglish (US)
Pages (from-to)652-654
Number of pages3
JournalJournal of Neurochemistry
Volume53
Issue number2
DOIs
StatePublished - Aug 1989

Keywords

  • Calcium
  • Dopamine
  • In vivo release
  • Ion‐selective microelectrodes
  • Microanalysis
  • α‐Methyl‐p‐tyrosine

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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