Ion transport across cat and ferret tracheal epithelia

R. J. Corrales, D. L. Coleman, David Jacoby, G. D. Leikauf, H. L. Hahn, J. A. Nadel, J. H. Widdicombe

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Sheets of trachea from ferret and cat were mounted in Ussing chambers and continuously short circuited. Under resting conditions, in both the cat and ferret there was little or no Cl secretion, and Na absorption accounted for most of the short-circuit current (I(sc)). Ouabain (10-4 M, serosal bath) reduced I(sc) to zero in 30-60 min. This decline was matched by a decrease in net Na absorption. Amiloride (10-4 M, luminal bath) caused a significant decrease in I(sc) and conductance (G) in both species. Bumetanide (10-4 M, serosal bath) had negligible effects on I(sc) and G. In both species, isoproterenol increased I(sc) by stimulating Cl secretion. Methacholine induced equal amounts of Na and Cl secretion, with little change in I(sc). In the cat, prostaglandins E2 and F(2α) and bradykinin increased I(sc), responses which were abolished in Cl-free medium. In open-circuited cat tissues, Na flux from the serosal to mucosal side was measured simultaneously with the secretion of nondialyzable 35S. Prostaglandins E1, E2, and F(2α), histamine, bradykinin, methacholine and isoproterenol all increased both Na and 35S-mucin secretion.

Original languageEnglish (US)
Pages (from-to)1065-1070
Number of pages6
JournalJournal of Applied Physiology
Volume61
Issue number3
StatePublished - 1986
Externally publishedYes

Fingerprint

Ferrets
Ion Transport
Cats
Epithelium
Baths
Methacholine Chloride
Prostaglandins F
Bradykinin
Isoproterenol
Dinoprostone
Bumetanide
Amiloride
Alprostadil
Mucins
Ouabain
Trachea
Histamine

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Corrales, R. J., Coleman, D. L., Jacoby, D., Leikauf, G. D., Hahn, H. L., Nadel, J. A., & Widdicombe, J. H. (1986). Ion transport across cat and ferret tracheal epithelia. Journal of Applied Physiology, 61(3), 1065-1070.

Ion transport across cat and ferret tracheal epithelia. / Corrales, R. J.; Coleman, D. L.; Jacoby, David; Leikauf, G. D.; Hahn, H. L.; Nadel, J. A.; Widdicombe, J. H.

In: Journal of Applied Physiology, Vol. 61, No. 3, 1986, p. 1065-1070.

Research output: Contribution to journalArticle

Corrales, RJ, Coleman, DL, Jacoby, D, Leikauf, GD, Hahn, HL, Nadel, JA & Widdicombe, JH 1986, 'Ion transport across cat and ferret tracheal epithelia', Journal of Applied Physiology, vol. 61, no. 3, pp. 1065-1070.
Corrales RJ, Coleman DL, Jacoby D, Leikauf GD, Hahn HL, Nadel JA et al. Ion transport across cat and ferret tracheal epithelia. Journal of Applied Physiology. 1986;61(3):1065-1070.
Corrales, R. J. ; Coleman, D. L. ; Jacoby, David ; Leikauf, G. D. ; Hahn, H. L. ; Nadel, J. A. ; Widdicombe, J. H. / Ion transport across cat and ferret tracheal epithelia. In: Journal of Applied Physiology. 1986 ; Vol. 61, No. 3. pp. 1065-1070.
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