TY - JOUR
T1 - Ion transport across cat and ferret tracheal epithelia
AU - Corrales, R. J.
AU - Coleman, D. L.
AU - Jacoby, D. B.
AU - Leikauf, G. D.
AU - Hahn, H. L.
AU - Nadel, J. A.
AU - Widdicombe, J. H.
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1986
Y1 - 1986
N2 - Sheets of trachea from ferret and cat were mounted in Ussing chambers and continuously short circuited. Under resting conditions, in both the cat and ferret there was little or no Cl secretion, and Na absorption accounted for most of the short-circuit current (I(sc)). Ouabain (10-4 M, serosal bath) reduced I(sc) to zero in 30-60 min. This decline was matched by a decrease in net Na absorption. Amiloride (10-4 M, luminal bath) caused a significant decrease in I(sc) and conductance (G) in both species. Bumetanide (10-4 M, serosal bath) had negligible effects on I(sc) and G. In both species, isoproterenol increased I(sc) by stimulating Cl secretion. Methacholine induced equal amounts of Na and Cl secretion, with little change in I(sc). In the cat, prostaglandins E2 and F(2α) and bradykinin increased I(sc), responses which were abolished in Cl-free medium. In open-circuited cat tissues, Na flux from the serosal to mucosal side was measured simultaneously with the secretion of nondialyzable 35S. Prostaglandins E1, E2, and F(2α), histamine, bradykinin, methacholine and isoproterenol all increased both Na and 35S-mucin secretion.
AB - Sheets of trachea from ferret and cat were mounted in Ussing chambers and continuously short circuited. Under resting conditions, in both the cat and ferret there was little or no Cl secretion, and Na absorption accounted for most of the short-circuit current (I(sc)). Ouabain (10-4 M, serosal bath) reduced I(sc) to zero in 30-60 min. This decline was matched by a decrease in net Na absorption. Amiloride (10-4 M, luminal bath) caused a significant decrease in I(sc) and conductance (G) in both species. Bumetanide (10-4 M, serosal bath) had negligible effects on I(sc) and G. In both species, isoproterenol increased I(sc) by stimulating Cl secretion. Methacholine induced equal amounts of Na and Cl secretion, with little change in I(sc). In the cat, prostaglandins E2 and F(2α) and bradykinin increased I(sc), responses which were abolished in Cl-free medium. In open-circuited cat tissues, Na flux from the serosal to mucosal side was measured simultaneously with the secretion of nondialyzable 35S. Prostaglandins E1, E2, and F(2α), histamine, bradykinin, methacholine and isoproterenol all increased both Na and 35S-mucin secretion.
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U2 - 10.1152/jappl.1986.61.3.1065
DO - 10.1152/jappl.1986.61.3.1065
M3 - Article
C2 - 3759745
AN - SCOPUS:0022512190
SN - 8750-7587
VL - 61
SP - 1065
EP - 1070
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
IS - 3
ER -