Involvement of caspase-3 in epigallocatechin-3-gallate-mediated apoptosis of human chondrosarcoma cells

Shabana Islam, Najmul Islam, Timothy Kermode, Brian Johnstone, Hasan Mukhtar, Roland W. Moskowitz, Victor M. Goldberg, Charles J. Malemud, Tariq M. Haqqi

Research output: Contribution to journalArticlepeer-review

115 Scopus citations

Abstract

Green tea polyphenol-(-)epigallocatechin-3-gallate (EGCG)-is a potent chemopreventive agent in many test systems and has been shown to inhibit tumor promotion and induce apoptosis. In this study we describe a novel observation that EGCG displayed strong inhibitory effects on the proliferation and viability of HTB-94 human chondrosarcoma cells in a dose-dependent manner and induced apoptosis. Investigation of the mechanism of EGCG-induced apoptosis revealed that treatment with EGCG resulted in DNA fragmentation, induction of caspase-3/CPP32 activity, and cleavage of the death substrate poly(ADP-ribose)polymerase (PARP). Pretreatment of cells with a synthetic pan-caspase inhibitor (Z-VAD FMR) and a caspase-3-specific inhibitor (DEVD-CHO) prevented EGCG-induced PARP cleavage. The induction of apoptosis by EGCG via activation of caspase-3/CPP32-like proteases may provide a mechanistic explanation for its antitumor effects. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)793-797
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume270
Issue number3
DOIs
StatePublished - Apr 21 2000

Keywords

  • Apoptosis
  • Caspases
  • Chondrosarcoma
  • Green tea

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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