Involvement of caspase-3 in epigallocatechin-3-gallate-mediated apoptosis of human chondrosarcoma cells

Shabana Islam, Najmul Islam, Timothy Kermode, Brian Johnstone, Hasan Mukhtar, Roland W. Moskowitz, Victor M. Goldberg, Charles J. Malemud, Tariq M. Haqqi

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Green tea polyphenol-(-)epigallocatechin-3-gallate (EGCG)-is a potent chemopreventive agent in many test systems and has been shown to inhibit tumor promotion and induce apoptosis. In this study we describe a novel observation that EGCG displayed strong inhibitory effects on the proliferation and viability of HTB-94 human chondrosarcoma cells in a dose-dependent manner and induced apoptosis. Investigation of the mechanism of EGCG-induced apoptosis revealed that treatment with EGCG resulted in DNA fragmentation, induction of caspase-3/CPP32 activity, and cleavage of the death substrate poly(ADP-ribose)polymerase (PARP). Pretreatment of cells with a synthetic pan-caspase inhibitor (Z-VAD FMR) and a caspase-3-specific inhibitor (DEVD-CHO) prevented EGCG-induced PARP cleavage. The induction of apoptosis by EGCG via activation of caspase-3/CPP32-like proteases may provide a mechanistic explanation for its antitumor effects. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)793-797
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume270
Issue number3
DOIs
StatePublished - Apr 21 2000
Externally publishedYes

Fingerprint

Chondrosarcoma
Caspase 3
Apoptosis
Poly(ADP-ribose) Polymerases
Ferromagnetic resonance
Caspase Inhibitors
Polyphenols
DNA Fragmentation
Tea
epigallocatechin gallate
Tumors
Peptide Hydrolases
Chemical activation
Cells
Observation
DNA
Substrates
Neoplasms

Keywords

  • Apoptosis
  • Caspases
  • Chondrosarcoma
  • Green tea

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Involvement of caspase-3 in epigallocatechin-3-gallate-mediated apoptosis of human chondrosarcoma cells. / Islam, Shabana; Islam, Najmul; Kermode, Timothy; Johnstone, Brian; Mukhtar, Hasan; Moskowitz, Roland W.; Goldberg, Victor M.; Malemud, Charles J.; Haqqi, Tariq M.

In: Biochemical and Biophysical Research Communications, Vol. 270, No. 3, 21.04.2000, p. 793-797.

Research output: Contribution to journalArticle

Islam, S, Islam, N, Kermode, T, Johnstone, B, Mukhtar, H, Moskowitz, RW, Goldberg, VM, Malemud, CJ & Haqqi, TM 2000, 'Involvement of caspase-3 in epigallocatechin-3-gallate-mediated apoptosis of human chondrosarcoma cells', Biochemical and Biophysical Research Communications, vol. 270, no. 3, pp. 793-797. https://doi.org/10.1006/bbrc.2000.2536
Islam, Shabana ; Islam, Najmul ; Kermode, Timothy ; Johnstone, Brian ; Mukhtar, Hasan ; Moskowitz, Roland W. ; Goldberg, Victor M. ; Malemud, Charles J. ; Haqqi, Tariq M. / Involvement of caspase-3 in epigallocatechin-3-gallate-mediated apoptosis of human chondrosarcoma cells. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 270, No. 3. pp. 793-797.
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