Investigation of choriocarcinoma clonal cell lines in vitro and choriocarcinoma transplants in the hamster for the secretion of a thyroid stimulating factor

J. M. Ketelslegers, B. C. Nisula, Peter Kohler

Research output: Contribution to journalArticle

Abstract

Six choriocarcinoma cell lines that secrete chorionic gonadotropin (hCG) in tissue culture were investigated for the production of thyroid stimulators. In the mouse thyrotropin bioassay, no thyroid stimulating activity was found in cell culture media even after 30 fold concentration by the method of Bates. Further, concentrates of culture media did not react in a porcine thyrotropin radioimmunoassay in which human chorionic thyrotropin cross reacted completely. Thyroid function was also assessed in hamsters bearing hCG secreting human choriocarcinoma transplants. Even though the thyroid stimulating factor present in commercial urinary hCG preparations caused an increase in protein bound 131I in the hamster, the presence of the choriocarcinoma had no effect on protein bound 131I. From these results, it appears that human choriocarcinoma cell lines maintained in tissue culture and human choriocarcinoma serially transplanted in the hamster are not suitable models for the study of the secretion of a thyroid stimulating factor by trophoblastic tissues.

Original languageEnglish (US)
Pages (from-to)808-810
Number of pages3
JournalEndocrinology
Volume96
Issue number3
StatePublished - 1975
Externally publishedYes

Fingerprint

Choriocarcinoma
Cricetinae
Thyroid Gland
Transplants
Cell Line
Thyrotropin
Culture Media
Thromboplastin
Chorionic Gonadotropin
Biological Assay
Radioimmunoassay
Proteins
Swine
Cell Culture Techniques
In Vitro Techniques

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Investigation of choriocarcinoma clonal cell lines in vitro and choriocarcinoma transplants in the hamster for the secretion of a thyroid stimulating factor. / Ketelslegers, J. M.; Nisula, B. C.; Kohler, Peter.

In: Endocrinology, Vol. 96, No. 3, 1975, p. 808-810.

Research output: Contribution to journalArticle

@article{8f9468c5853941e09e4e1e688d2d58db,
title = "Investigation of choriocarcinoma clonal cell lines in vitro and choriocarcinoma transplants in the hamster for the secretion of a thyroid stimulating factor",
abstract = "Six choriocarcinoma cell lines that secrete chorionic gonadotropin (hCG) in tissue culture were investigated for the production of thyroid stimulators. In the mouse thyrotropin bioassay, no thyroid stimulating activity was found in cell culture media even after 30 fold concentration by the method of Bates. Further, concentrates of culture media did not react in a porcine thyrotropin radioimmunoassay in which human chorionic thyrotropin cross reacted completely. Thyroid function was also assessed in hamsters bearing hCG secreting human choriocarcinoma transplants. Even though the thyroid stimulating factor present in commercial urinary hCG preparations caused an increase in protein bound 131I in the hamster, the presence of the choriocarcinoma had no effect on protein bound 131I. From these results, it appears that human choriocarcinoma cell lines maintained in tissue culture and human choriocarcinoma serially transplanted in the hamster are not suitable models for the study of the secretion of a thyroid stimulating factor by trophoblastic tissues.",
author = "Ketelslegers, {J. M.} and Nisula, {B. C.} and Peter Kohler",
year = "1975",
language = "English (US)",
volume = "96",
pages = "808--810",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "3",

}

TY - JOUR

T1 - Investigation of choriocarcinoma clonal cell lines in vitro and choriocarcinoma transplants in the hamster for the secretion of a thyroid stimulating factor

AU - Ketelslegers, J. M.

AU - Nisula, B. C.

AU - Kohler, Peter

PY - 1975

Y1 - 1975

N2 - Six choriocarcinoma cell lines that secrete chorionic gonadotropin (hCG) in tissue culture were investigated for the production of thyroid stimulators. In the mouse thyrotropin bioassay, no thyroid stimulating activity was found in cell culture media even after 30 fold concentration by the method of Bates. Further, concentrates of culture media did not react in a porcine thyrotropin radioimmunoassay in which human chorionic thyrotropin cross reacted completely. Thyroid function was also assessed in hamsters bearing hCG secreting human choriocarcinoma transplants. Even though the thyroid stimulating factor present in commercial urinary hCG preparations caused an increase in protein bound 131I in the hamster, the presence of the choriocarcinoma had no effect on protein bound 131I. From these results, it appears that human choriocarcinoma cell lines maintained in tissue culture and human choriocarcinoma serially transplanted in the hamster are not suitable models for the study of the secretion of a thyroid stimulating factor by trophoblastic tissues.

AB - Six choriocarcinoma cell lines that secrete chorionic gonadotropin (hCG) in tissue culture were investigated for the production of thyroid stimulators. In the mouse thyrotropin bioassay, no thyroid stimulating activity was found in cell culture media even after 30 fold concentration by the method of Bates. Further, concentrates of culture media did not react in a porcine thyrotropin radioimmunoassay in which human chorionic thyrotropin cross reacted completely. Thyroid function was also assessed in hamsters bearing hCG secreting human choriocarcinoma transplants. Even though the thyroid stimulating factor present in commercial urinary hCG preparations caused an increase in protein bound 131I in the hamster, the presence of the choriocarcinoma had no effect on protein bound 131I. From these results, it appears that human choriocarcinoma cell lines maintained in tissue culture and human choriocarcinoma serially transplanted in the hamster are not suitable models for the study of the secretion of a thyroid stimulating factor by trophoblastic tissues.

UR - http://www.scopus.com/inward/record.url?scp=0016639346&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016639346&partnerID=8YFLogxK

M3 - Article

C2 - 1167827

AN - SCOPUS:0016639346

VL - 96

SP - 808

EP - 810

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 3

ER -