Invasion and replication of Salmonella typhimurium in animal cells

L. C. Gahring, Fred Heffron, B. B. Finlay, S. Falkow

Research output: Contribution to journalArticle

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Abstract

A total of 81 avirulent Tn10 insertion mutants of Salmonella typhimurium have previously been described. These mutants were selected for the inability to survive in murine macrophages. We have characterized the abilities of the most avirulent of these mutants to adhere to, invade, and replicate in both macrophages and nonphagocytic epithelial cells. The results suggest that most mutants contain a defect that is specific to survival within professional phagocytes. These mutants invaded and replicated normally within nonphagocytic human colon adenocarcinoma cells (Caco-2) but did not survive in the macrophage cell line J774. One mutant invaded both macrophages and epithelial cells much less efficiently than the parental strain. The defect associated with this mutant appears to be a result of decreased adherence to animal cells.

Original languageEnglish (US)
Pages (from-to)443-448
Number of pages6
JournalInfection and Immunity
Volume58
Issue number2
StatePublished - 1990
Externally publishedYes

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Salmonella typhimurium
Macrophages
Epithelial Cells
Caco-2 Cells
Phagocytes
Colon
Adenocarcinoma
Cell Line
Survival

ASJC Scopus subject areas

  • Immunology

Cite this

Gahring, L. C., Heffron, F., Finlay, B. B., & Falkow, S. (1990). Invasion and replication of Salmonella typhimurium in animal cells. Infection and Immunity, 58(2), 443-448.

Invasion and replication of Salmonella typhimurium in animal cells. / Gahring, L. C.; Heffron, Fred; Finlay, B. B.; Falkow, S.

In: Infection and Immunity, Vol. 58, No. 2, 1990, p. 443-448.

Research output: Contribution to journalArticle

Gahring, LC, Heffron, F, Finlay, BB & Falkow, S 1990, 'Invasion and replication of Salmonella typhimurium in animal cells', Infection and Immunity, vol. 58, no. 2, pp. 443-448.
Gahring LC, Heffron F, Finlay BB, Falkow S. Invasion and replication of Salmonella typhimurium in animal cells. Infection and Immunity. 1990;58(2):443-448.
Gahring, L. C. ; Heffron, Fred ; Finlay, B. B. ; Falkow, S. / Invasion and replication of Salmonella typhimurium in animal cells. In: Infection and Immunity. 1990 ; Vol. 58, No. 2. pp. 443-448.
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