Invasion and replication of Salmonella typhimurium in animal cells

L. C. Gahring, F. Heffron, B. B. Finlay, S. Falkow

    Research output: Contribution to journalArticlepeer-review

    78 Scopus citations


    A total of 81 avirulent Tn10 insertion mutants of Salmonella typhimurium have previously been described. These mutants were selected for the inability to survive in murine macrophages. We have characterized the abilities of the most avirulent of these mutants to adhere to, invade, and replicate in both macrophages and nonphagocytic epithelial cells. The results suggest that most mutants contain a defect that is specific to survival within professional phagocytes. These mutants invaded and replicated normally within nonphagocytic human colon adenocarcinoma cells (Caco-2) but did not survive in the macrophage cell line J774. One mutant invaded both macrophages and epithelial cells much less efficiently than the parental strain. The defect associated with this mutant appears to be a result of decreased adherence to animal cells.

    Original languageEnglish (US)
    Pages (from-to)443-448
    Number of pages6
    JournalInfection and Immunity
    Issue number2
    StatePublished - 1990

    ASJC Scopus subject areas

    • Parasitology
    • Microbiology
    • Immunology
    • Infectious Diseases


    Dive into the research topics of 'Invasion and replication of Salmonella typhimurium in animal cells'. Together they form a unique fingerprint.

    Cite this