Intracellular transport of herpes simplex virus gD occurs more rapidly in uninfected cells than in infected cells

David Johnson, J. R. Smiley

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A mouse L cell line which expresses the herpex simplex virus type 1 immediate-early polypeptides ICP4 and ICP47 was cotransfected with a cloned copy of the Bg/II L fragment of herpes simplex virus type 2, which includes the gene for gD, and the plasmid pSV2neo, which contains the aminoglycosyl 3'-phosphotransferase (agpt) gene conferring resistance to the antibiotic G418. A G418-resistant transformed cell line was isolated which expressed herpes simplex virus type 2 gD at higher levels than were found in infected cells. The intracellular transport and processing of gD was compared in transformed and infected cells. In the transformed Z4/6 cells gD was rapidly processed and transported to the cell surface; in contrast, the processing and cell surface appearance of gD in infected parental Z4 cells occurred at a much slower rate, and gD accumulated in nuclear membrane to a greater extent. Thus, the movement of HSV-2 gD to the cell surface in infected cells is retarded as viral glycoproteins accumulate in the nuclear envelope, probably because they interact with other viral structural components.

Original languageEnglish (US)
Pages (from-to)682-689
Number of pages8
JournalJournal of Virology
Volume54
Issue number3
StatePublished - 1985
Externally publishedYes

Fingerprint

herpes simplex
Simplexvirus
viruses
Human Herpesvirus 2
Human herpesvirus 2
cells
Nuclear Envelope
nuclear membrane
geneticin
cell lines
Viral Structures
Transformed Cell Line
Microbial Drug Resistance
Genes
glycoproteins
polypeptides
Glycoproteins
plasmids
phosphotransferases (kinases)
Plasmids

ASJC Scopus subject areas

  • Immunology

Cite this

Intracellular transport of herpes simplex virus gD occurs more rapidly in uninfected cells than in infected cells. / Johnson, David; Smiley, J. R.

In: Journal of Virology, Vol. 54, No. 3, 1985, p. 682-689.

Research output: Contribution to journalArticle

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