Intracellular processing of epidermal growth factor. II. Intracellular cleavage of the COOH-terminal region of 125I-epidermal growth factor

Stephen Planck, J. S. Finch, B. E. Magun

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Epidermal growth factor (EGF) undergoes a specific series of alterations during the course of its binding and internalization into cultured fibroblasts. The modified EGF species can be distinguished from each other and from native EGF by their isoelectric points. We employed peptide mapping techniques to determine the nature of these alterations. We found that 125I-EGF with a pI of 4.55 was converted to a pI 4.2 species by removal of 1 or 2 amino acid moieties from the COOH-terminal end of the protein. A pI 4.35 species was generated by a trypsin-like cut between amino acid residues 48 and 49, for a total of 5 amino acid moieties removed from the native EGF. The pI 4.0 species was formed by removal of at least the COOH-terminal arginine from the pI 4.35 species. Thus, upon binding and internalization, EGF was sequentially cleaved in the COOH-terminal region. Removal of the COOH-terminal polypeptide has been shown to dramatically reduce the affinity of EGF for its receptor, raising the possibility that intracellular dissociation of EGF from its receptor may be a direct result of the intracellular processing of EGF.

Original languageEnglish (US)
Pages (from-to)3053-3057
Number of pages5
JournalJournal of Biological Chemistry
Volume259
Issue number5
StatePublished - 1984
Externally publishedYes

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Epidermal Growth Factor
Processing
Epidermal Growth Factor Receptor
Amino Acids
Peptides
Peptide Mapping
Isoelectric Point
Fibroblasts
Trypsin
Arginine
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Intracellular processing of epidermal growth factor. II. Intracellular cleavage of the COOH-terminal region of 125I-epidermal growth factor. / Planck, Stephen; Finch, J. S.; Magun, B. E.

In: Journal of Biological Chemistry, Vol. 259, No. 5, 1984, p. 3053-3057.

Research output: Contribution to journalArticle

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