Intracellular pH in human fibroblasts

effect of mitogens, A23187, and phospholipase activation.

Leslie Muldoon, R. J. Dinerstein, M. L. Villereal

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Serum-deprived human fibroblasts (HSWP cells) were loaded with either the fluorescent pH indicator 6-carboxy-4',5'-dimethylfluorescein or the calcium indicator quin 2, and the fluorescence of the intracellular probes was continuously monitored with a microspectrofluorometer. Addition of a cocktail of peptide growth factors causes intracellular alkalinization, which is blocked by amiloride or by replacement of extracellular Na+ with choline, confirming that mitogenic stimulation activates a Na+-H+ exchanger in HSWP cells. The exchanger is also activated by A23187, acid loading the cells, and stimulation of phospholipase activity with melittin. Growth factors and melittin activation of the exchanger have been demonstrated to be dependent on the mobilization of Ca2+ from intracellular stores. The intracellular alkalization and increase in Ca2+ activity due to both melittin and growth factors is inhibited by phospholipase inhibitors, indicating that phospholipase activity is necessary for the activation of the Na+-H+ exchanger and the mobilization of intracellular Ca2+.

Original languageEnglish (US)
JournalThe American journal of physiology
Volume249
Issue number1 Pt 1
StatePublished - Jul 1985
Externally publishedYes

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Melitten
Phospholipases
Calcimycin
Mitogens
Intercellular Signaling Peptides and Proteins
Sodium-Hydrogen Antiporter
Fibroblasts
Amiloride
Choline
Fluorescence
Calcium
Peptides
Acids
Serum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Intracellular pH in human fibroblasts : effect of mitogens, A23187, and phospholipase activation. / Muldoon, Leslie; Dinerstein, R. J.; Villereal, M. L.

In: The American journal of physiology, Vol. 249, No. 1 Pt 1, 07.1985.

Research output: Contribution to journalArticle

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