Intracellular imaging of HCV RNA and cellular lipids by using simultaneous two-photon fluorescence and coherent anti-stokes Raman scattering microscopies

Xiaolin Nan, Angela M. Tonary, Albert Stolow, X. Sunney Xie, John Paul Pezacki

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Hepatitis C virus (HCV) infection is associated with changes in host-cell lipid metabolism. Here we describe a new approach for detecting HCV RNA using two-photon fluorescence (TPF), and HCV-associated changes in cellular lipids using coherent anti-Stokes Raman scattering (CARS) microscopy. By combining the two types of microscopy with a common laser source, we visualized both phenomena simultaneously and profiled cellular lipids and subcellular localization of RNA in real time.

Original languageEnglish (US)
Pages (from-to)1895-1897
Number of pages3
JournalChemBioChem
Volume7
Issue number12
DOIs
StatePublished - Dec 2006
Externally publishedYes

Fingerprint

Raman Spectrum Analysis
Photons
Viruses
Hepacivirus
Raman scattering
Microscopy
Microscopic examination
Fluorescence
RNA
Lipids
Imaging techniques
Virus Diseases
Lipid Metabolism
Lasers

Keywords

  • CARS microscopy
  • Hepatitis C virus
  • Host-viral interactions
  • RNA
  • Two-photon fluorescence

ASJC Scopus subject areas

  • Drug Discovery
  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Intracellular imaging of HCV RNA and cellular lipids by using simultaneous two-photon fluorescence and coherent anti-stokes Raman scattering microscopies. / Nan, Xiaolin; Tonary, Angela M.; Stolow, Albert; Xie, X. Sunney; Pezacki, John Paul.

In: ChemBioChem, Vol. 7, No. 12, 12.2006, p. 1895-1897.

Research output: Contribution to journalArticle

Nan, Xiaolin ; Tonary, Angela M. ; Stolow, Albert ; Xie, X. Sunney ; Pezacki, John Paul. / Intracellular imaging of HCV RNA and cellular lipids by using simultaneous two-photon fluorescence and coherent anti-stokes Raman scattering microscopies. In: ChemBioChem. 2006 ; Vol. 7, No. 12. pp. 1895-1897.
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