Abstract
Hepatitis C virus (HCV) infection is associated with changes in host-cell lipid metabolism. Here we describe a new approach for detecting HCV RNA using two-photon fluorescence (TPF), and HCV-associated changes in cellular lipids using coherent anti-Stokes Raman scattering (CARS) microscopy. By combining the two types of microscopy with a common laser source, we visualized both phenomena simultaneously and profiled cellular lipids and subcellular localization of RNA in real time.
Original language | English (US) |
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Pages (from-to) | 1895-1897 |
Number of pages | 3 |
Journal | ChemBioChem |
Volume | 7 |
Issue number | 12 |
DOIs | |
State | Published - Dec 2006 |
Externally published | Yes |
Keywords
- CARS microscopy
- Hepatitis C virus
- Host-viral interactions
- RNA
- Two-photon fluorescence
ASJC Scopus subject areas
- Biochemistry
- Molecular Medicine
- Molecular Biology
- Organic Chemistry