Intracellular imaging of HCV RNA and cellular lipids by using simultaneous two-photon fluorescence and coherent anti-stokes Raman scattering microscopies

Xiaolin Nan, Angela M. Tonary, Albert Stolow, X. Sunney Xie, John Paul Pezacki

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

Hepatitis C virus (HCV) infection is associated with changes in host-cell lipid metabolism. Here we describe a new approach for detecting HCV RNA using two-photon fluorescence (TPF), and HCV-associated changes in cellular lipids using coherent anti-Stokes Raman scattering (CARS) microscopy. By combining the two types of microscopy with a common laser source, we visualized both phenomena simultaneously and profiled cellular lipids and subcellular localization of RNA in real time.

Original languageEnglish (US)
Pages (from-to)1895-1897
Number of pages3
JournalChemBioChem
Volume7
Issue number12
DOIs
StatePublished - Dec 2006

Keywords

  • CARS microscopy
  • Hepatitis C virus
  • Host-viral interactions
  • RNA
  • Two-photon fluorescence

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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