Intracellular HMGB1 as a novel tumor suppressor of pancreatic cancer

Rui Kang, Yangchun Xie, Qiuhong Zhang, Wen Hou, Qingping Jiang, Shan Zhu, Jinbao Liu, Dexing Zeng, Haichao Wang, David L. Bartlett, Timothy R. Billiar, Herbert J. Zeh, Michael T. Lotze, Daolin Tang

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Pancreatic ductal adenocarcinoma (PDAC) driven by oncogenic K-Ras remains among the most lethal human cancers despite recent advances in modern medicine. The pathogenesis of PDAC is partly attributable to intrinsic chromosome instability and extrinsic inflammation activation. However, the molecular link between these two events in pancreatic tumorigenesis has not yet been fully established. Here, we show that intracellular high mobility group box 1 (HMGB1) remarkably suppresses oncogenic K-Ras-driven pancreatic tumorigenesis by inhibiting chromosome instability-mediated pro-inflammatory nucleosome release. Conditional genetic ablation of either single or both alleles of HMGB1 in the pancreas renders mice extremely sensitive to oncogenic K-Ras-driven initiation of precursor lesions at birth, including pancreatic intraepithelial neoplasms, intraductal papillary mucinous neoplasms, and mucinous cystic neoplasms. Loss of HMGB1 in the pancreas is associated with oxidative DNA damage and chromosomal instability characterized by chromosome rearrangements and telomere abnormalities. These lead to inflammatory nucleosome release and propagate K-Ras-driven pancreatic tumorigenesis. Extracellular nucleosomes promote interleukin 6 (IL-6) secretion by infiltrating macrophages/neutrophils and enhance oncogenic K-Ras signaling activation in pancreatic lesions. Neutralizing antibodies to IL-6 or histone H3 or knockout of the receptor for advanced glycation end products all limit K-Ras signaling activation, prevent cancer development and metastasis/invasion, and prolong animal survival in Pdx1-Cre;K-Ras G12D/+;Hmgb1-/- mice. Pharmacological inhibition of HMGB1 loss by glycyrrhizin limits oncogenic K-Ras-driven tumorigenesis in mice under inflammatory conditions. Diminished nuclear and total cellular expression of HMGB1 in PDAC patients correlates with poor overall survival, supporting intracellular HMGB1 as a novel tumor suppressor with prognostic and therapeutic relevance in PDAC.

Original languageEnglish (US)
Pages (from-to)916-932
Number of pages17
JournalCell Research
Volume27
Issue number7
DOIs
StatePublished - Jul 1 2017
Externally publishedYes

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Pancreatic Neoplasms
Chromosomal Instability
Nucleosomes
Carcinogenesis
Adenocarcinoma
Neoplasms
Pancreas
Interleukin-6
Glycyrrhizic Acid
Modern 1601-history
Carcinoma in Situ
Telomere
Neutralizing Antibodies
Histones
DNA Damage
Neutrophils
Chromosomes
Alleles
Macrophages
Parturition

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Kang, R., Xie, Y., Zhang, Q., Hou, W., Jiang, Q., Zhu, S., ... Tang, D. (2017). Intracellular HMGB1 as a novel tumor suppressor of pancreatic cancer. Cell Research, 27(7), 916-932. https://doi.org/10.1038/cr.2017.51

Intracellular HMGB1 as a novel tumor suppressor of pancreatic cancer. / Kang, Rui; Xie, Yangchun; Zhang, Qiuhong; Hou, Wen; Jiang, Qingping; Zhu, Shan; Liu, Jinbao; Zeng, Dexing; Wang, Haichao; Bartlett, David L.; Billiar, Timothy R.; Zeh, Herbert J.; Lotze, Michael T.; Tang, Daolin.

In: Cell Research, Vol. 27, No. 7, 01.07.2017, p. 916-932.

Research output: Contribution to journalArticle

Kang, R, Xie, Y, Zhang, Q, Hou, W, Jiang, Q, Zhu, S, Liu, J, Zeng, D, Wang, H, Bartlett, DL, Billiar, TR, Zeh, HJ, Lotze, MT & Tang, D 2017, 'Intracellular HMGB1 as a novel tumor suppressor of pancreatic cancer', Cell Research, vol. 27, no. 7, pp. 916-932. https://doi.org/10.1038/cr.2017.51
Kang R, Xie Y, Zhang Q, Hou W, Jiang Q, Zhu S et al. Intracellular HMGB1 as a novel tumor suppressor of pancreatic cancer. Cell Research. 2017 Jul 1;27(7):916-932. https://doi.org/10.1038/cr.2017.51
Kang, Rui ; Xie, Yangchun ; Zhang, Qiuhong ; Hou, Wen ; Jiang, Qingping ; Zhu, Shan ; Liu, Jinbao ; Zeng, Dexing ; Wang, Haichao ; Bartlett, David L. ; Billiar, Timothy R. ; Zeh, Herbert J. ; Lotze, Michael T. ; Tang, Daolin. / Intracellular HMGB1 as a novel tumor suppressor of pancreatic cancer. In: Cell Research. 2017 ; Vol. 27, No. 7. pp. 916-932.
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abstract = "Pancreatic ductal adenocarcinoma (PDAC) driven by oncogenic K-Ras remains among the most lethal human cancers despite recent advances in modern medicine. The pathogenesis of PDAC is partly attributable to intrinsic chromosome instability and extrinsic inflammation activation. However, the molecular link between these two events in pancreatic tumorigenesis has not yet been fully established. Here, we show that intracellular high mobility group box 1 (HMGB1) remarkably suppresses oncogenic K-Ras-driven pancreatic tumorigenesis by inhibiting chromosome instability-mediated pro-inflammatory nucleosome release. Conditional genetic ablation of either single or both alleles of HMGB1 in the pancreas renders mice extremely sensitive to oncogenic K-Ras-driven initiation of precursor lesions at birth, including pancreatic intraepithelial neoplasms, intraductal papillary mucinous neoplasms, and mucinous cystic neoplasms. Loss of HMGB1 in the pancreas is associated with oxidative DNA damage and chromosomal instability characterized by chromosome rearrangements and telomere abnormalities. These lead to inflammatory nucleosome release and propagate K-Ras-driven pancreatic tumorigenesis. Extracellular nucleosomes promote interleukin 6 (IL-6) secretion by infiltrating macrophages/neutrophils and enhance oncogenic K-Ras signaling activation in pancreatic lesions. Neutralizing antibodies to IL-6 or histone H3 or knockout of the receptor for advanced glycation end products all limit K-Ras signaling activation, prevent cancer development and metastasis/invasion, and prolong animal survival in Pdx1-Cre;K-Ras G12D/+;Hmgb1-/- mice. Pharmacological inhibition of HMGB1 loss by glycyrrhizin limits oncogenic K-Ras-driven tumorigenesis in mice under inflammatory conditions. Diminished nuclear and total cellular expression of HMGB1 in PDAC patients correlates with poor overall survival, supporting intracellular HMGB1 as a novel tumor suppressor with prognostic and therapeutic relevance in PDAC.",
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