Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium

Steven L. Jacques, Randolph D. Glickman, Jon A. Schwartz

Research output: Chapter in Book/Report/Conference proceedingConference contribution

66 Scopus citations

Abstract

This study determine the threshold radiant exposure from a 10-ns pulsed laser (532 nm) which caused bovine melanosomes to present various observable endpoints of disruption. The endpoints tested were (1) a visible region of clearing in a uniform field of melanosomes under a glass cover slip, (2) an audible sound, and (3) the increase in melanin photoreactivity due to photodisruption of melanosomes. The thresholds were tested for different starting temperatures by pre-equilibrating the melanosomes in aqueous solution at various temperatures. Lower temperatures required larger exposures to attain a given endpoint. The data suggest that bovine RPE melanosomes are about 4-fold denser in melanin content than cutaneous melanosomes.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsSteven L. Jacques
Pages468-477
Number of pages10
StatePublished - Jan 1 1996
EventLaser-Tissue Interaction VII - San Jose, CA, USA
Duration: Jan 29 1996Feb 1 1996

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume2681

Other

OtherLaser-Tissue Interaction VII
CitySan Jose, CA, USA
Period1/29/962/1/96

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Jacques, S. L., Glickman, R. D., & Schwartz, J. A. (1996). Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium. In S. L. Jacques (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (pp. 468-477). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 2681).