Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium

Steven Jacques, Randolph D. Glickman, Jon A. Schwartz

Research output: Chapter in Book/Report/Conference proceedingConference contribution

61 Citations (Scopus)

Abstract

This study determine the threshold radiant exposure from a 10-ns pulsed laser (532 nm) which caused bovine melanosomes to present various observable endpoints of disruption. The endpoints tested were (1) a visible region of clearing in a uniform field of melanosomes under a glass cover slip, (2) an audible sound, and (3) the increase in melanin photoreactivity due to photodisruption of melanosomes. The thresholds were tested for different starting temperatures by pre-equilibrating the melanosomes in aqueous solution at various temperatures. Lower temperatures required larger exposures to attain a given endpoint. The data suggest that bovine RPE melanosomes are about 4-fold denser in melanin content than cutaneous melanosomes.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsSteven L. Jacques
Pages468-477
Number of pages10
Volume2681
StatePublished - 1996
Externally publishedYes
EventLaser-Tissue Interaction VII - San Jose, CA, USA
Duration: Jan 29 1996Feb 1 1996

Other

OtherLaser-Tissue Interaction VII
CitySan Jose, CA, USA
Period1/29/962/1/96

Fingerprint

Pulsed lasers
Pigments
Melanin
Photoreactivity
Temperature
Acoustic waves
Glass

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Jacques, S., Glickman, R. D., & Schwartz, J. A. (1996). Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium. In S. L. Jacques (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2681, pp. 468-477)

Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium. / Jacques, Steven; Glickman, Randolph D.; Schwartz, Jon A.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / Steven L. Jacques. Vol. 2681 1996. p. 468-477.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Jacques, S, Glickman, RD & Schwartz, JA 1996, Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium. in SL Jacques (ed.), Proceedings of SPIE - The International Society for Optical Engineering. vol. 2681, pp. 468-477, Laser-Tissue Interaction VII, San Jose, CA, USA, 1/29/96.
Jacques S, Glickman RD, Schwartz JA. Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium. In Jacques SL, editor, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2681. 1996. p. 468-477
Jacques, Steven ; Glickman, Randolph D. ; Schwartz, Jon A. / Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium. Proceedings of SPIE - The International Society for Optical Engineering. editor / Steven L. Jacques. Vol. 2681 1996. pp. 468-477
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