Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium

Steven L. Jacques; Randolph D. Glickman; Jon A. Schwartz

doi:10.1117/12.239608

Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium

Steven L. Jacques, Randolph D. Glickman, Jon A. Schwartz

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

81 Scopus citations

Abstract

This study determine the threshold radiant exposure from a 10-ns pulsed laser (532 nm) which caused bovine melanosomes to present various observable endpoints of disruption. The endpoints tested were (1) a visible region of clearing in a uniform field of melanosomes under a glass cover slip, (2) an audible sound, and (3) the increase in melanin photoreactivity due to photodisruption of melanosomes. The thresholds were tested for different starting temperatures by pre-equilibrating the melanosomes in aqueous solution at various temperatures. Lower temperatures required larger exposures to attain a given endpoint. The data suggest that bovine RPE melanosomes are about 4-fold denser in melanin content than cutaneous melanosomes.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Pages	468-477
Number of pages	10
Volume	2681
DOIs	https://doi.org/10.1117/12.239608
State	Published - Jan 1 1996
Externally published	Yes
Event	Laser-Tissue Interaction VII - San Jose, CA, USA Duration: Jan 29 1996 → Feb 1 1996

Other

Other	Laser-Tissue Interaction VII
City	San Jose, CA, USA
Period	1/29/96 → 2/1/96

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.239608

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Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium. / Jacques, Steven L.; Glickman, Randolph D.; Schwartz, Jon A.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2681 1996. p. 468-477.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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title = "Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium",

abstract = "This study determine the threshold radiant exposure from a 10-ns pulsed laser (532 nm) which caused bovine melanosomes to present various observable endpoints of disruption. The endpoints tested were (1) a visible region of clearing in a uniform field of melanosomes under a glass cover slip, (2) an audible sound, and (3) the increase in melanin photoreactivity due to photodisruption of melanosomes. The thresholds were tested for different starting temperatures by pre-equilibrating the melanosomes in aqueous solution at various temperatures. Lower temperatures required larger exposures to attain a given endpoint. The data suggest that bovine RPE melanosomes are about 4-fold denser in melanin content than cutaneous melanosomes.",

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N2 - This study determine the threshold radiant exposure from a 10-ns pulsed laser (532 nm) which caused bovine melanosomes to present various observable endpoints of disruption. The endpoints tested were (1) a visible region of clearing in a uniform field of melanosomes under a glass cover slip, (2) an audible sound, and (3) the increase in melanin photoreactivity due to photodisruption of melanosomes. The thresholds were tested for different starting temperatures by pre-equilibrating the melanosomes in aqueous solution at various temperatures. Lower temperatures required larger exposures to attain a given endpoint. The data suggest that bovine RPE melanosomes are about 4-fold denser in melanin content than cutaneous melanosomes.

AB - This study determine the threshold radiant exposure from a 10-ns pulsed laser (532 nm) which caused bovine melanosomes to present various observable endpoints of disruption. The endpoints tested were (1) a visible region of clearing in a uniform field of melanosomes under a glass cover slip, (2) an audible sound, and (3) the increase in melanin photoreactivity due to photodisruption of melanosomes. The thresholds were tested for different starting temperatures by pre-equilibrating the melanosomes in aqueous solution at various temperatures. Lower temperatures required larger exposures to attain a given endpoint. The data suggest that bovine RPE melanosomes are about 4-fold denser in melanin content than cutaneous melanosomes.

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BT - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Laser-Tissue Interaction VII

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Internal absorption coefficient and threshold for pulsed laser disruption of melanosomes isolated from retinal pigment epithelium

Abstract

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