Interleukin-20 receptor expression in the trabecular meshwork and its implication in glaucoma

Kate Keller, Yong Feng Yang, Ying Ying Sun, Renee Sykes, Natalie Donna Gaudette, John R. Samples, Ted Acott, Mary Wirtz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Purpose: To determine whether interleukin-20 receptors (IL-20R) are expressed in trabecular meshwork cells and the effect of a T104M mutation in IL-20R2 on downstream cellular functions. Methods: Evaluation of signal transducer and activator of transcription (STAT)3 phosphorylation and generic matrix metalloproteinase (MMP) activity in primary open angle glaucoma (POAG) dermal fibroblasts (pHDF) with the T104M IL-20R2 mutation were compared with normal human dermal fibroblasts (HDF). Expression of IL-20R1 and IL-20R2 in human trabecular meshwork (HTM) cells was determined by immunohistochemistry and western immunoblotting. Results: A T104M mutation in IL20-R2 was identified in a large POAG family in which the GLC1C locus was originally mapped. pHDFs harboring this mutation had significantly increased phosphorylated STAT3 (pSTAT3) activity compared with normal HDFs. However, stimulation with either IL-19 or IL-20 for 15min resulted in significantly decreased levels of pSTAT3 in pHDFs compared with controls. Generic MMP activity was significantly decreased in pHDFs compared with controls after stimulation with IL-20 for 24h. Both IL-20R1 and IL-20R2 receptors were expressed in HTM cells by western immunoblot and immunofluorescence, and they appeared to be up-regulated in response to cytokine treatment. Conclusions: A T104M mutation in IL-20R2 significantly impacts the function of this receptor as shown by decreased pSTAT3 levels and generic MMP activity. Reduced MMP activity may affect the ability of glaucoma patients to alter outflow resistance in response to elevated intraocular pressure.

Original languageEnglish (US)
Pages (from-to)267-276
Number of pages10
JournalJournal of Ocular Pharmacology and Therapeutics
Volume30
Issue number2-3
DOIs
StatePublished - Mar 1 2014

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Trabecular Meshwork
Glaucoma
Matrix Metalloproteinases
Mutation
Fibroblasts
Western Blotting
STAT3 Transcription Factor
Skin
Aptitude
Intraocular Pressure
Fluorescent Antibody Technique
Immunohistochemistry
Phosphorylation
interleukin-20 receptor
Cytokines

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Ophthalmology
  • Pharmacology
  • Medicine(all)

Cite this

Interleukin-20 receptor expression in the trabecular meshwork and its implication in glaucoma. / Keller, Kate; Yang, Yong Feng; Sun, Ying Ying; Sykes, Renee; Gaudette, Natalie Donna; Samples, John R.; Acott, Ted; Wirtz, Mary.

In: Journal of Ocular Pharmacology and Therapeutics, Vol. 30, No. 2-3, 01.03.2014, p. 267-276.

Research output: Contribution to journalArticle

Keller, Kate ; Yang, Yong Feng ; Sun, Ying Ying ; Sykes, Renee ; Gaudette, Natalie Donna ; Samples, John R. ; Acott, Ted ; Wirtz, Mary. / Interleukin-20 receptor expression in the trabecular meshwork and its implication in glaucoma. In: Journal of Ocular Pharmacology and Therapeutics. 2014 ; Vol. 30, No. 2-3. pp. 267-276.
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AU - Samples, John R.

AU - Acott, Ted

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