Interleukin-2 activation of STAT5 requires the convergent action of tyrosine kinases and a serine/threonine kinase pathway distinct from the Raf1/ERK2 MAP kinase pathway

Carol Beadling, Julian Ng, Jane W. Babbage, Doreen A. Cantrell

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

Interleukin-2 (IL-2) induces DNA binding of STAT5, a member of the family of cytokine-regulated transcription factors termed 'signal transducers and activators of transcription'. IL-2-stimulated STAT5-DNA complexes include two tyrosine phosphoproteins which exhibit distinct mobilities in SDS-PAGE gels. Our studies have shown that IL-2 rapidly induces both tyrosine phosphorylation and serine phosphorylation of STAT5 and that the two STAT5 tyrosine phosphoproteins detected in IL-2-activated cells differ in their levels of phosphorylation on serine residues. The two different phosphoforms of STAT5 have identical in vitro DNA binding specificity and reactivity with tyrosine phosphopeptides, but differ in their cellular localization. As well, the present data indicate that the transcriptional activity of STAT5 is regulated by serine kinases in T lymphocytes. Two previously characterized serine kinases activated by IL-2, MAP kinase/ERK2 and p70 S6 kinase, do not appear to be involved in STAT5 regulation by this cytokine. Accordingly, STAT5 activation in T cells requires the convergent action of tyrosine kinases and a distinct serine/threonine kinase which has not previously been implicated in IL-2 signalling.

Original languageEnglish (US)
Pages (from-to)1902-1913
Number of pages12
JournalEMBO Journal
Volume15
Issue number8
StatePublished - Apr 15 1996
Externally publishedYes

Fingerprint

Protein-Serine-Threonine Kinases
Protein-Tyrosine Kinases
Interleukin-2
Phosphotransferases
Chemical activation
Phosphorylation
Tyrosine
T-cells
Phosphoproteins
Serine
DNA
Cytokines
70-kDa Ribosomal Protein S6 Kinases
T-Lymphocytes
Phosphopeptides
Transcription
Transducers
Polyacrylamide Gel Electrophoresis
Transcription Factors
Gels

Keywords

  • Interleukin-2
  • Signal transduction
  • STAT5

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Interleukin-2 activation of STAT5 requires the convergent action of tyrosine kinases and a serine/threonine kinase pathway distinct from the Raf1/ERK2 MAP kinase pathway. / Beadling, Carol; Ng, Julian; Babbage, Jane W.; Cantrell, Doreen A.

In: EMBO Journal, Vol. 15, No. 8, 15.04.1996, p. 1902-1913.

Research output: Contribution to journalArticle

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AU - Cantrell, Doreen A.

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