Interferon regulatory factor 3 is necessary for induction of antiviral genes during human cytomegalovirus infection

Victor R. DeFilippis, Bridget Robinson, Thomas M. Keck, Scott G. Hansen, Jay A. Nelson, Klaus J. Früh

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Viral infection activates interferon regulatory factor 3 (IRF3), a cofactor for the induction of interferon-stimulated genes (ISGs). The role of IRF3 in the activation of ISGs by human cytomegalovirus (HCMV) is controversial despite the fact that HCMV has consistently been shown to induce ISGs during infection of fibroblasts. To address the function of IRF3 in HCMV-mediated ISG induction, we monitored ISG expression and global gene expression in HCMV-infected cells in which IRF3 function had been depleted by small interfering RNA or blocked by dominant negative IRF3. A specific reduction of ISG induction was observed, whereas other transcripts were unaffected. We therefore conclude that IRF3 specifically regulates ISG induction during the initial phase of HCMV infection.

Original languageEnglish (US)
Pages (from-to)1032-1037
Number of pages6
JournalJournal of virology
Volume80
Issue number2
DOIs
StatePublished - Jan 2006

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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