Interaction of the regulatory subunit (RII) of cAMP-dependent protein kinase with RII-anchoring proteins occurs through an amphipathic helix binding motif

Daniel Carr, Renata E. Stofko-Hahn, Iain D C Fraser, Sarah M. Bishop, Ted Acott, Richard G. Brennan, John D. Scott

Research output: Contribution to journalArticle

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Abstract

The type II cAMP-dependent protein kinase is localized to specific subcellular environments through the binding of the regulatory subunit (RII) dimer to RII-anchoring proteins. Computer-aided analysis of secondary structure, performed on four RII-anchoring protein sequences (the microtubule-associated protein 2, P150, and two thyroid proteins Ht 21 and Ht 31), has identified common regions of approximately 14 residues which display high probabilities of forming amphipathic helices. The potential amphipathic helix region of Ht 31 (Leu-Ile-Glu-Glu-Ala-Ala-Ser-Arg-Ile-Val-Asp-Ala-Val-Ile) lies between residues 494 and 507. A bacterially expressed 318-amino acid fragment, Ht 31 (418-736), containing the amphipathic helix region, was able to bind RIIα. Site-directed mutagenesis designed to disrupt the secondary structure in the putative binding helix reduced binding dramatically. Specifically, substitution of proline for Ala-498 significantly diminished RIIα binding, and similar mutation of Ile-502 or Ile-507 abolished interaction. Mutation of Ala-522 to proline, which is located outside the predicted amphipathic helix region, had no effect on RIIα binding. These data suggest that anchoring proteins interact with RIIα via an amphipathic helix binding motif.

Original languageEnglish (US)
Pages (from-to)14188-14192
Number of pages5
JournalJournal of Biological Chemistry
Volume266
Issue number22
StatePublished - 1991

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Cyclic AMP-Dependent Protein Kinases
Proline
Proteins
Computer aided analysis
Mutagenesis
Mutation
Microtubule-Associated Proteins
Site-Directed Mutagenesis
Dimers
Thyroid Gland
Substitution reactions
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

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Interaction of the regulatory subunit (RII) of cAMP-dependent protein kinase with RII-anchoring proteins occurs through an amphipathic helix binding motif. / Carr, Daniel; Stofko-Hahn, Renata E.; Fraser, Iain D C; Bishop, Sarah M.; Acott, Ted; Brennan, Richard G.; Scott, John D.

In: Journal of Biological Chemistry, Vol. 266, No. 22, 1991, p. 14188-14192.

Research output: Contribution to journalArticle

Carr, Daniel ; Stofko-Hahn, Renata E. ; Fraser, Iain D C ; Bishop, Sarah M. ; Acott, Ted ; Brennan, Richard G. ; Scott, John D. / Interaction of the regulatory subunit (RII) of cAMP-dependent protein kinase with RII-anchoring proteins occurs through an amphipathic helix binding motif. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 22. pp. 14188-14192.
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