Interaction of specific nuclear factors with the nuclease-hypersensitive region of the chicken adult β-globin gene: Nature of the binding domain

Beverly Emerson, Catherine D. Lewis, Gary Felsenfeld

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

We have studied the properties of a factor or factors that bind selectively to the 5′ flanking region of the chicken adult β-globin (βA-globin) gene. We previously showed that these components, when bound with histones on plasmids containing the region, confer on the complex a pattern of hypersensitivity to nuclease digestion similar to that in the nucleus. We have now measured the abundance of the factor(s) in partially purified preparations, and the affinity constants for binding to specific and nonspecific DNA sequences. Footprinting studies of the specific complex with DNAase I and II reveal two discrete protected regions within the hypersensitive domain. When these regions are physically separated, they interact with the factor(s) independently, suggesting that each region binds one or more distinct components. The footprint patterns of our complexes in vitro agree with the patterns observed in intact chicken erythrocyte nuclei. These complexes thus are on the transcriptionally active βA-globin gene in vivo.

Original languageEnglish (US)
Pages (from-to)21-30
Number of pages10
JournalCell
Volume41
Issue number1
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

Fingerprint

Globins
Chickens
Genes
5' Flanking Region
Deoxyribonuclease I
DNA sequences
Histones
Digestion
Hypersensitivity
Plasmids
Erythrocytes
In Vitro Techniques
deoxyribonuclease II

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Interaction of specific nuclear factors with the nuclease-hypersensitive region of the chicken adult β-globin gene : Nature of the binding domain. / Emerson, Beverly; Lewis, Catherine D.; Felsenfeld, Gary.

In: Cell, Vol. 41, No. 1, 01.01.1985, p. 21-30.

Research output: Contribution to journalArticle

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