Interaction among beta-endorphin, nitric oxide and prostaglandins during ovulation in rats

Alicia G. Faletti, C. Mohn, M. Farina, Alejandro Lomniczi, V. Rettori

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The aim of this study was to investigate the relationship between β-endorphin and nitric oxide (NO) during the ovulatory process in rats. Immature rats were treated with equine chorionic gonadotrophin-hCG to induce ovulation. An intrabursal injection of β-endorphin stimulated nitric oxide synthase (NOS) activity. This effect was completely reversed when naltrexone was co-injected with β-endorphin. The stimulatory action of β-endorphin on NOS activity was studied to determine whether it was exerted via prostaglandins. Treatment with prostaglandin E2 (PGE2) completely reversed the β-endorphin-induced stimulation of NOS activity. Moreover, intrabursal injection of meloxicam, an inhibitor of cyclooxygenase 2, increased NOS activity, but this effect was not altered by co-injection with β-endorphin. The presence of both endothelial NOS (eNOS) and inducible NOS (iNOS) in the ovary at 10 h after hCG treatment was studied by western blot analysis. Local administration of β-endorphin inhibited the expression of eNOS protein, whereas expression of iNOS protein was not detectable. Ovarian β-endorphin content was diminished at 10 h after hCG injection. Treatment with prostaglandin synthesis inhibitors in vivo augmented the ovarian β-endorphin content. In conclusion, these results indicate that β-endorphin stimulates the activity of ovarian NOS indirectly by inhibiting prostaglandin production.

Original languageEnglish (US)
Pages (from-to)469-477
Number of pages9
JournalReproduction
Volume125
Issue number4
StatePublished - Apr 1 2003
Externally publishedYes

Fingerprint

Endorphins
beta-Endorphin
Ovulation
Prostaglandins
Nitric Oxide
Nitric Oxide Synthase
Injections
meloxicam
Nitric Oxide Synthase Type II
Equine Gonadotropins
Prostaglandin Antagonists
Naltrexone
Nitric Oxide Synthase Type III
Cyclooxygenase 2 Inhibitors
Chorionic Gonadotropin
Dinoprostone
Ovary
Therapeutics
Western Blotting

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Cell Biology
  • Endocrinology
  • Embryology

Cite this

Faletti, A. G., Mohn, C., Farina, M., Lomniczi, A., & Rettori, V. (2003). Interaction among beta-endorphin, nitric oxide and prostaglandins during ovulation in rats. Reproduction, 125(4), 469-477.

Interaction among beta-endorphin, nitric oxide and prostaglandins during ovulation in rats. / Faletti, Alicia G.; Mohn, C.; Farina, M.; Lomniczi, Alejandro; Rettori, V.

In: Reproduction, Vol. 125, No. 4, 01.04.2003, p. 469-477.

Research output: Contribution to journalArticle

Faletti, AG, Mohn, C, Farina, M, Lomniczi, A & Rettori, V 2003, 'Interaction among beta-endorphin, nitric oxide and prostaglandins during ovulation in rats', Reproduction, vol. 125, no. 4, pp. 469-477.
Faletti, Alicia G. ; Mohn, C. ; Farina, M. ; Lomniczi, Alejandro ; Rettori, V. / Interaction among beta-endorphin, nitric oxide and prostaglandins during ovulation in rats. In: Reproduction. 2003 ; Vol. 125, No. 4. pp. 469-477.
@article{3f53621a112c43b39465d30f98fde4ae,
title = "Interaction among beta-endorphin, nitric oxide and prostaglandins during ovulation in rats",
abstract = "The aim of this study was to investigate the relationship between β-endorphin and nitric oxide (NO) during the ovulatory process in rats. Immature rats were treated with equine chorionic gonadotrophin-hCG to induce ovulation. An intrabursal injection of β-endorphin stimulated nitric oxide synthase (NOS) activity. This effect was completely reversed when naltrexone was co-injected with β-endorphin. The stimulatory action of β-endorphin on NOS activity was studied to determine whether it was exerted via prostaglandins. Treatment with prostaglandin E2 (PGE2) completely reversed the β-endorphin-induced stimulation of NOS activity. Moreover, intrabursal injection of meloxicam, an inhibitor of cyclooxygenase 2, increased NOS activity, but this effect was not altered by co-injection with β-endorphin. The presence of both endothelial NOS (eNOS) and inducible NOS (iNOS) in the ovary at 10 h after hCG treatment was studied by western blot analysis. Local administration of β-endorphin inhibited the expression of eNOS protein, whereas expression of iNOS protein was not detectable. Ovarian β-endorphin content was diminished at 10 h after hCG injection. Treatment with prostaglandin synthesis inhibitors in vivo augmented the ovarian β-endorphin content. In conclusion, these results indicate that β-endorphin stimulates the activity of ovarian NOS indirectly by inhibiting prostaglandin production.",
author = "Faletti, {Alicia G.} and C. Mohn and M. Farina and Alejandro Lomniczi and V. Rettori",
year = "2003",
month = "4",
day = "1",
language = "English (US)",
volume = "125",
pages = "469--477",
journal = "Reproduction",
issn = "1470-1626",
publisher = "BioScientifica Ltd.",
number = "4",

}

TY - JOUR

T1 - Interaction among beta-endorphin, nitric oxide and prostaglandins during ovulation in rats

AU - Faletti, Alicia G.

AU - Mohn, C.

AU - Farina, M.

AU - Lomniczi, Alejandro

AU - Rettori, V.

PY - 2003/4/1

Y1 - 2003/4/1

N2 - The aim of this study was to investigate the relationship between β-endorphin and nitric oxide (NO) during the ovulatory process in rats. Immature rats were treated with equine chorionic gonadotrophin-hCG to induce ovulation. An intrabursal injection of β-endorphin stimulated nitric oxide synthase (NOS) activity. This effect was completely reversed when naltrexone was co-injected with β-endorphin. The stimulatory action of β-endorphin on NOS activity was studied to determine whether it was exerted via prostaglandins. Treatment with prostaglandin E2 (PGE2) completely reversed the β-endorphin-induced stimulation of NOS activity. Moreover, intrabursal injection of meloxicam, an inhibitor of cyclooxygenase 2, increased NOS activity, but this effect was not altered by co-injection with β-endorphin. The presence of both endothelial NOS (eNOS) and inducible NOS (iNOS) in the ovary at 10 h after hCG treatment was studied by western blot analysis. Local administration of β-endorphin inhibited the expression of eNOS protein, whereas expression of iNOS protein was not detectable. Ovarian β-endorphin content was diminished at 10 h after hCG injection. Treatment with prostaglandin synthesis inhibitors in vivo augmented the ovarian β-endorphin content. In conclusion, these results indicate that β-endorphin stimulates the activity of ovarian NOS indirectly by inhibiting prostaglandin production.

AB - The aim of this study was to investigate the relationship between β-endorphin and nitric oxide (NO) during the ovulatory process in rats. Immature rats were treated with equine chorionic gonadotrophin-hCG to induce ovulation. An intrabursal injection of β-endorphin stimulated nitric oxide synthase (NOS) activity. This effect was completely reversed when naltrexone was co-injected with β-endorphin. The stimulatory action of β-endorphin on NOS activity was studied to determine whether it was exerted via prostaglandins. Treatment with prostaglandin E2 (PGE2) completely reversed the β-endorphin-induced stimulation of NOS activity. Moreover, intrabursal injection of meloxicam, an inhibitor of cyclooxygenase 2, increased NOS activity, but this effect was not altered by co-injection with β-endorphin. The presence of both endothelial NOS (eNOS) and inducible NOS (iNOS) in the ovary at 10 h after hCG treatment was studied by western blot analysis. Local administration of β-endorphin inhibited the expression of eNOS protein, whereas expression of iNOS protein was not detectable. Ovarian β-endorphin content was diminished at 10 h after hCG injection. Treatment with prostaglandin synthesis inhibitors in vivo augmented the ovarian β-endorphin content. In conclusion, these results indicate that β-endorphin stimulates the activity of ovarian NOS indirectly by inhibiting prostaglandin production.

UR - http://www.scopus.com/inward/record.url?scp=0037396954&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037396954&partnerID=8YFLogxK

M3 - Article

C2 - 12683918

AN - SCOPUS:0037396954

VL - 125

SP - 469

EP - 477

JO - Reproduction

JF - Reproduction

SN - 1470-1626

IS - 4

ER -