Integration of Golgi trafficking and growth factor signaling by the lipid phosphatase SAC1

Anastasia Blagoveshchenskaya, Ying Cheong Fei, Holger M. Rohde, Greta Glover, Andreas Knödler, Teresa Nicolson, Guido Boehmelt, Peter Mayinger

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Abstract

When a growing cell expands, lipids and proteins must be delivered to its periphery. Although this phenomenon has been observed for decades, it remains unknown how the secretory pathway responds to growth signaling. We demonstrate that control of Golgi phosphatidylinositol-4-phosphate (PI(4)P) is required for growth-dependent secretion. The phosphoinositide phosphatase SAC1 accumulates at the Golgi in quiescent cells and down-regulates anterograde trafficking by depleting Golgi PI(4)P. Golgi localization requires oligomerization of SAC1 and recruitment of the coat protein (COP) II complex. When quiescent cells are stimulated by mitogens, SAC1 rapidly shuttles back to the endoplasmic reticulum (ER), thus releasing the brake on Golgi secretion. The p38 mitogen-activated kinase (MAPK) pathway induces dissociation of SAC1 oligomers after mitogen stimulation, which triggers COP-I-mediated retrieval of SAC1 to the ER. Inhibition of p38 MAPK abolishes growth factor-induced Golgi-to-ER shuttling of SAC1 and slows secretion. These results suggest direct roles for p38 MAPK and SAC1 in transmitting growth signals to the secretory machinery.

Original languageEnglish (US)
Pages (from-to)803-812
Number of pages10
JournalJournal of Cell Biology
Volume180
Issue number4
DOIs
StatePublished - Feb 25 2008

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Mitogens
Phosphoric Monoester Hydrolases
Endoplasmic Reticulum
Intercellular Signaling Peptides and Proteins
Capsid Proteins
p38 Mitogen-Activated Protein Kinases
Lipids
Growth
Secretory Pathway
Mitogen-Activated Protein Kinase Kinases
Down-Regulation
Proteins

ASJC Scopus subject areas

  • Cell Biology

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Integration of Golgi trafficking and growth factor signaling by the lipid phosphatase SAC1. / Blagoveshchenskaya, Anastasia; Fei, Ying Cheong; Rohde, Holger M.; Glover, Greta; Knödler, Andreas; Nicolson, Teresa; Boehmelt, Guido; Mayinger, Peter.

In: Journal of Cell Biology, Vol. 180, No. 4, 25.02.2008, p. 803-812.

Research output: Contribution to journalArticle

Blagoveshchenskaya, A, Fei, YC, Rohde, HM, Glover, G, Knödler, A, Nicolson, T, Boehmelt, G & Mayinger, P 2008, 'Integration of Golgi trafficking and growth factor signaling by the lipid phosphatase SAC1', Journal of Cell Biology, vol. 180, no. 4, pp. 803-812. https://doi.org/10.1083/jcb.200708109
Blagoveshchenskaya, Anastasia ; Fei, Ying Cheong ; Rohde, Holger M. ; Glover, Greta ; Knödler, Andreas ; Nicolson, Teresa ; Boehmelt, Guido ; Mayinger, Peter. / Integration of Golgi trafficking and growth factor signaling by the lipid phosphatase SAC1. In: Journal of Cell Biology. 2008 ; Vol. 180, No. 4. pp. 803-812.
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