Insulin-like growth factor I promotes leiomyoma cell growth in vitro

Estil Y. Strawn, Miles J. Novy, Kenneth A. Burry, Cynthia L. Bethea

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    68 Scopus citations

    Abstract

    Objective: Our purpose was to determine whether insulin-like growth factors I and II preferentially stimulate uterine leiomyoma cells versus myometrial cells in monolayer culture. Study design: Leiomyomas and normal myometrium were obtained at hysterectomy from five premenopausal women. Specimens were enzymatically digested for use in primary monolayer cell cultures. By use of serum-free media, insulin-like growth factor I or II was added in 1, 10, and 100 ng/ml concentrations to both cell types with the patient serving as her own control. Cell number, prolactin production, and proliferative index values were measured on day 15 of cell culture. Results: Significant increases in cell number were found in the leiomyoma cultures (p < 0.05) treated with 10 and 100 ng/ml insulin-like growth factors I but not with insulin-like growth factos II. Neither factor exerted a stimulatory effect on myometrial cells. Conclusion: Insulin-like growth factor sI preferentially stimulates leiomyoma cells in monolayer culture. These results suggest an autocrine-paracine role in vivo for this factor in conjuction with gonadal steroids in promoting leiomyoma growth.

    Original languageEnglish (US)
    Pages (from-to)1837-1844
    Number of pages8
    JournalAmerican journal of obstetrics and gynecology
    Volume172
    Issue number6
    DOIs
    StatePublished - Jan 1 1995

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    Keywords

    • Insulin-like growth factor-1
    • uterine myoma in vitro

    ASJC Scopus subject areas

    • Obstetrics and Gynecology

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