Insulin-like growth factor I promotes leiomyoma cell growth in vitro

Estil Y. Strawn, Miles J. Novy, Kenneth A. Burry, Cynthia Bethea

    Research output: Contribution to journalArticle

    68 Citations (Scopus)

    Abstract

    Objective: Our purpose was to determine whether insulin-like growth factors I and II preferentially stimulate uterine leiomyoma cells versus myometrial cells in monolayer culture. Study design: Leiomyomas and normal myometrium were obtained at hysterectomy from five premenopausal women. Specimens were enzymatically digested for use in primary monolayer cell cultures. By use of serum-free media, insulin-like growth factor I or II was added in 1, 10, and 100 ng/ml concentrations to both cell types with the patient serving as her own control. Cell number, prolactin production, and proliferative index values were measured on day 15 of cell culture. Results: Significant increases in cell number were found in the leiomyoma cultures (p <0.05) treated with 10 and 100 ng/ml insulin-like growth factors I but not with insulin-like growth factors II. Neither factor exerted a stimulatory effect on myometrial cells. Conclusion: Insulin-like growth factors I preferentially stimulates leiomyoma cells in monolayer culture. These results suggest an autocrine-paracrine role in vivo for this factor in conjunction with gonadal steroids in promoting leiomyoma growth.

    Original languageEnglish (US)
    Pages (from-to)1837-1844
    Number of pages8
    JournalAmerican Journal of Obstetrics and Gynecology
    Volume172
    Issue number6
    DOIs
    StatePublished - 1995

    Fingerprint

    Leiomyoma
    Insulin-Like Growth Factor I
    Insulin-Like Growth Factor II
    Growth
    Cell Count
    Primary Cell Culture
    Myometrium
    Serum-Free Culture Media
    Hysterectomy
    Prolactin
    Cell Culture Techniques
    Steroids
    In Vitro Techniques

    Keywords

    • insulin-like growth factor-I
    • uterine myoma in vitro

    ASJC Scopus subject areas

    • Medicine(all)
    • Obstetrics and Gynecology

    Cite this

    Insulin-like growth factor I promotes leiomyoma cell growth in vitro. / Strawn, Estil Y.; Novy, Miles J.; Burry, Kenneth A.; Bethea, Cynthia.

    In: American Journal of Obstetrics and Gynecology, Vol. 172, No. 6, 1995, p. 1837-1844.

    Research output: Contribution to journalArticle

    Strawn, Estil Y. ; Novy, Miles J. ; Burry, Kenneth A. ; Bethea, Cynthia. / Insulin-like growth factor I promotes leiomyoma cell growth in vitro. In: American Journal of Obstetrics and Gynecology. 1995 ; Vol. 172, No. 6. pp. 1837-1844.
    @article{0fd915fe6d384c4ca04fa25e17817c40,
    title = "Insulin-like growth factor I promotes leiomyoma cell growth in vitro",
    abstract = "Objective: Our purpose was to determine whether insulin-like growth factors I and II preferentially stimulate uterine leiomyoma cells versus myometrial cells in monolayer culture. Study design: Leiomyomas and normal myometrium were obtained at hysterectomy from five premenopausal women. Specimens were enzymatically digested for use in primary monolayer cell cultures. By use of serum-free media, insulin-like growth factor I or II was added in 1, 10, and 100 ng/ml concentrations to both cell types with the patient serving as her own control. Cell number, prolactin production, and proliferative index values were measured on day 15 of cell culture. Results: Significant increases in cell number were found in the leiomyoma cultures (p <0.05) treated with 10 and 100 ng/ml insulin-like growth factors I but not with insulin-like growth factors II. Neither factor exerted a stimulatory effect on myometrial cells. Conclusion: Insulin-like growth factors I preferentially stimulates leiomyoma cells in monolayer culture. These results suggest an autocrine-paracrine role in vivo for this factor in conjunction with gonadal steroids in promoting leiomyoma growth.",
    keywords = "insulin-like growth factor-I, uterine myoma in vitro",
    author = "Strawn, {Estil Y.} and Novy, {Miles J.} and Burry, {Kenneth A.} and Cynthia Bethea",
    year = "1995",
    doi = "10.1016/0002-9378(95)91420-X",
    language = "English (US)",
    volume = "172",
    pages = "1837--1844",
    journal = "American Journal of Obstetrics and Gynecology",
    issn = "0002-9378",
    publisher = "Mosby Inc.",
    number = "6",

    }

    TY - JOUR

    T1 - Insulin-like growth factor I promotes leiomyoma cell growth in vitro

    AU - Strawn, Estil Y.

    AU - Novy, Miles J.

    AU - Burry, Kenneth A.

    AU - Bethea, Cynthia

    PY - 1995

    Y1 - 1995

    N2 - Objective: Our purpose was to determine whether insulin-like growth factors I and II preferentially stimulate uterine leiomyoma cells versus myometrial cells in monolayer culture. Study design: Leiomyomas and normal myometrium were obtained at hysterectomy from five premenopausal women. Specimens were enzymatically digested for use in primary monolayer cell cultures. By use of serum-free media, insulin-like growth factor I or II was added in 1, 10, and 100 ng/ml concentrations to both cell types with the patient serving as her own control. Cell number, prolactin production, and proliferative index values were measured on day 15 of cell culture. Results: Significant increases in cell number were found in the leiomyoma cultures (p <0.05) treated with 10 and 100 ng/ml insulin-like growth factors I but not with insulin-like growth factors II. Neither factor exerted a stimulatory effect on myometrial cells. Conclusion: Insulin-like growth factors I preferentially stimulates leiomyoma cells in monolayer culture. These results suggest an autocrine-paracrine role in vivo for this factor in conjunction with gonadal steroids in promoting leiomyoma growth.

    AB - Objective: Our purpose was to determine whether insulin-like growth factors I and II preferentially stimulate uterine leiomyoma cells versus myometrial cells in monolayer culture. Study design: Leiomyomas and normal myometrium were obtained at hysterectomy from five premenopausal women. Specimens were enzymatically digested for use in primary monolayer cell cultures. By use of serum-free media, insulin-like growth factor I or II was added in 1, 10, and 100 ng/ml concentrations to both cell types with the patient serving as her own control. Cell number, prolactin production, and proliferative index values were measured on day 15 of cell culture. Results: Significant increases in cell number were found in the leiomyoma cultures (p <0.05) treated with 10 and 100 ng/ml insulin-like growth factors I but not with insulin-like growth factors II. Neither factor exerted a stimulatory effect on myometrial cells. Conclusion: Insulin-like growth factors I preferentially stimulates leiomyoma cells in monolayer culture. These results suggest an autocrine-paracrine role in vivo for this factor in conjunction with gonadal steroids in promoting leiomyoma growth.

    KW - insulin-like growth factor-I

    KW - uterine myoma in vitro

    UR - http://www.scopus.com/inward/record.url?scp=0029036589&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0029036589&partnerID=8YFLogxK

    U2 - 10.1016/0002-9378(95)91420-X

    DO - 10.1016/0002-9378(95)91420-X

    M3 - Article

    C2 - 7778641

    AN - SCOPUS:0029036589

    VL - 172

    SP - 1837

    EP - 1844

    JO - American Journal of Obstetrics and Gynecology

    JF - American Journal of Obstetrics and Gynecology

    SN - 0002-9378

    IS - 6

    ER -