Insulin excites anorexigenic proopiomelanocortin neurons via activation of canonical transient receptor potential channels

Jian Qiu, Chunguang Zhang, Amanda Borgquist, Casey C. Nestor, Arik W. Smith, Martha A. Bosch, Stephen Ku, Edward J. Wagner, Oline Ronnekleiv, Martin Kelly

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Proopiomelanocortin (POMC) neurons within the hypothalamic arcuate nucleus are vital anorexigenic neurons. Although both the leptin and insulin receptors are coupled to the activation of phosphatidylinositide 3 kinase (PI3K) in POMC neurons, they are thought to have disparate actions on POMC excitability. Using whole-cell recording and selective pharmacological tools, we have found that, similar to leptin, purified insulin depolarized POMC and adjacent kisspeptin neurons via activation of TRPC5 channels, which are highly expressed in these neurons. In contrast, insulin hyperpolarized and inhibited NPY/AgRP neurons via activation of KATP channels. Moreover, Zn2+, which is found in insulin formulations at nanomolar concentrations, inhibited POMC neurons via activation of KATP channels. Finally, as predicted, insulin given intracerebroventrically robustly inhibited food intake and activated c-fos expression in arcuate POMC neurons. Our results show that purified insulin excites POMC neurons in the arcuate nucleus, which we propose is a major mechanism by which insulin regulates energy homeostasis.

Original languageEnglish (US)
Pages (from-to)682-693
Number of pages12
JournalCell Metabolism
Volume19
Issue number4
DOIs
StatePublished - Apr 1 2014

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Transient Receptor Potential Channels
Pro-Opiomelanocortin
Insulin
Neurons
KATP Channels
Arcuate Nucleus of Hypothalamus
Kisspeptins
Leptin Receptors
Insulin Receptor
Patch-Clamp Techniques
Leptin
Homeostasis
Phosphotransferases
Eating
Pharmacology

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Physiology
  • Medicine(all)

Cite this

Insulin excites anorexigenic proopiomelanocortin neurons via activation of canonical transient receptor potential channels. / Qiu, Jian; Zhang, Chunguang; Borgquist, Amanda; Nestor, Casey C.; Smith, Arik W.; Bosch, Martha A.; Ku, Stephen; Wagner, Edward J.; Ronnekleiv, Oline; Kelly, Martin.

In: Cell Metabolism, Vol. 19, No. 4, 01.04.2014, p. 682-693.

Research output: Contribution to journalArticle

Qiu, Jian ; Zhang, Chunguang ; Borgquist, Amanda ; Nestor, Casey C. ; Smith, Arik W. ; Bosch, Martha A. ; Ku, Stephen ; Wagner, Edward J. ; Ronnekleiv, Oline ; Kelly, Martin. / Insulin excites anorexigenic proopiomelanocortin neurons via activation of canonical transient receptor potential channels. In: Cell Metabolism. 2014 ; Vol. 19, No. 4. pp. 682-693.
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