Insights into the role of IL-12B and IFN-γ cytokine gene polymorphisms in HIV-1/AIDS infection

Ranbir Chander Sobti, A. M. Salih, B. Nega, A. H. Seyed, K. Rupinder, K. Vijesh, Ajay Wanchu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

One of the main characteristics of HIV-1 infection is persistent systemic immune activation. This immune activation and dysregulation is characterized by a specific pattern of cytokine production, expression of membrane activation molecules on the cells of the immune system, and changes in the levels of several immune parameters in blood. Therefore, the aim of the present work was to evaluate the effect of a Taq1 polymorphism in the 3′UTR of the IL-12B gene at position -1188 (A/C) and the biallelic polymorphism in the first intron of IFN-γ at position +874 (T/A) on HIV-1/AIDS among north Indian population. IL-12B and IFN-γ gene polymorphisms were studied in 300 patients with HIV-1/AIDS and an equal number of negatively diagnosed controls of the matched age, using DNA-based polymerase chain reaction with sequence-specific primers and restriction digestion. The allelic as well as genotypic frequencies of interleukin-12B gene polymorphisms did not significantly differ between HIV-1/AIDS patients and negative healthy controls. A statistically significant correlation was found between IFN-γ polymorphism and the risk of the disease. The present study suggested that individuals with mutant homozygous IFN-γ AA genotype were at risk of HIV-1/AIDS (OR = 1.88, 95% CI 1.14-3.10, P = 0.008).

Original languageEnglish (US)
Pages (from-to)110-115
Number of pages6
JournalFolia Biologica
Volume56
Issue number3
StatePublished - 2010
Externally publishedYes

Fingerprint

Polymorphism
HIV-1
Acquired Immunodeficiency Syndrome
Genes
Cytokines
Infection
Chemical activation
Interleukin-12 Subunit p40
Gene Order
Immune system
Polymerase chain reaction
DNA-Directed DNA Polymerase
Introns
HIV Infections
Digestion
Immune System
Blood
Genotype
Membranes
Polymerase Chain Reaction

Keywords

  • AIDS
  • ARMS
  • Cytokine
  • HIV-1
  • IFN-γ
  • IL-12B
  • Polymorphism
  • Pro-inflammatory
  • RFLP

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Biochemistry
  • Immunology
  • Developmental Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Sobti, R. C., Salih, A. M., Nega, B., Seyed, A. H., Rupinder, K., Vijesh, K., & Wanchu, A. (2010). Insights into the role of IL-12B and IFN-γ cytokine gene polymorphisms in HIV-1/AIDS infection. Folia Biologica, 56(3), 110-115.

Insights into the role of IL-12B and IFN-γ cytokine gene polymorphisms in HIV-1/AIDS infection. / Sobti, Ranbir Chander; Salih, A. M.; Nega, B.; Seyed, A. H.; Rupinder, K.; Vijesh, K.; Wanchu, Ajay.

In: Folia Biologica, Vol. 56, No. 3, 2010, p. 110-115.

Research output: Contribution to journalArticle

Sobti, RC, Salih, AM, Nega, B, Seyed, AH, Rupinder, K, Vijesh, K & Wanchu, A 2010, 'Insights into the role of IL-12B and IFN-γ cytokine gene polymorphisms in HIV-1/AIDS infection', Folia Biologica, vol. 56, no. 3, pp. 110-115.
Sobti RC, Salih AM, Nega B, Seyed AH, Rupinder K, Vijesh K et al. Insights into the role of IL-12B and IFN-γ cytokine gene polymorphisms in HIV-1/AIDS infection. Folia Biologica. 2010;56(3):110-115.
Sobti, Ranbir Chander ; Salih, A. M. ; Nega, B. ; Seyed, A. H. ; Rupinder, K. ; Vijesh, K. ; Wanchu, Ajay. / Insights into the role of IL-12B and IFN-γ cytokine gene polymorphisms in HIV-1/AIDS infection. In: Folia Biologica. 2010 ; Vol. 56, No. 3. pp. 110-115.
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