INS-fOCT: A label-free, all-optical method for simultaneously manipulating and mapping brain function

Ying Zhang, Lin Yao, Fen Yang, Shanshan Yang, Akshay Edathodathil, Wang Xi, Anna Wang Roe, Peng Li

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Significance: Current approaches to stimulating and recording from the brain have combined electrical or optogenetic stimulation with recording approaches, such as two-photon, electrophysiology (EP), and optical intrinsic signal imaging (OISI). However, we lack a label-free, all-optical approach with high spatial and temporal resolution. Aim: To develop a label-free, all-optical method that simultaneously manipulates and images brain function using pulsed near-infrared light (INS) and functional optical coherence tomography (fOCT), respectively. Approach: We built a coregistered INS, fOCT, and OISI system. OISI and EP recordings were employed to validate the fOCT signals. Results: The fOCT signal was reliable and regional, and the area of fOCT signal corresponded with the INS-activated region. The fOCT signal was in synchrony with the INS onset time with a delay of âˆ1/430 ms. The magnitude of fOCT signal exhibited a linear correlation with the INS radiant exposure. The significant correlation between the fOCT signal and INS was further supported by OISI and EP recordings. Conclusions: The proposed fiber-based, all-optical INS-fOCT method allows simultaneous stimulation and mapping without the risk of interchannel cross-talk and the requirement of contrast injection and viral transfection and offers a deep penetration depth and high resolution.

Original languageEnglish (US)
Article number015014
Issue number1
StatePublished - Jan 1 2020


  • functional imaging
  • functional optical coherence tomography
  • infrared neural stimulation

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging


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