Inhibition of the human intermediate conductance Ca2+-activated K+ channel, hIK1, by volatile anesthetics

Tsunehisa Namba, Takahiro M. Ishii, Mitsuko Ikeda, Taizo Hisano, Tatsuya Itoh, Kiichi Hirota, John P. Adelman, Kazuhiko Fukuda

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Ca2+-activated K+ channels (K(Ca)) regulate a wide variety of cellular functions by coupling intracellular Ca2+ concentration to membrane potential. There are three major groups of K(Ca) classified by their unit conductances: large (BK), intermediate (IK), and small (SK) conductance of channels. BK channel is gated by combined influences of Ca2+ and voltage, while IK and SK channels are gated solely by Ca2+. Volatile anesthetics inhibit BK channel activity by interfering with the Ca2+ gating mechanism. However, the effects of anesthetics on IK and SK channels are unknown. Using cloned IK and SK channels, hIK1 and hSK1-3, respectively, we found that the currents of hIK1 were inhibited rapidly and reversibly by volatile anesthetics, whereas those of SK channels were not affected. The IC50 values of the volatile anesthetics, halothane, sevoflurane, enflurane, and isoflurane for hIK1 inhibition were 0.69, 0.42, 1.01 and 1.03 mM, respectively, and were in the clinically used concentration range. In contrast to BK channel, halothane inhibition of hIK1 currents was independent of Ca2+ concentration, suggesting that Ca2+ gating mechanism is not involved. These results demonstrate that volatile anesthetics, such as halothane, enflurane, isoflurane, and sevoflurane, affect BK, IK, and SK channels in distinct ways. Copyright (C) 2000.

Original languageEnglish (US)
Pages (from-to)95-101
Number of pages7
JournalEuropean Journal of Pharmacology
Volume395
Issue number2
DOIs
StatePublished - Apr 28 2000

Keywords

  • Anesthetic
  • Ca-activated
  • K channel
  • Xenopus oocyte
  • volatile

ASJC Scopus subject areas

  • Pharmacology

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    Namba, T., Ishii, T. M., Ikeda, M., Hisano, T., Itoh, T., Hirota, K., Adelman, J. P., & Fukuda, K. (2000). Inhibition of the human intermediate conductance Ca2+-activated K+ channel, hIK1, by volatile anesthetics. European Journal of Pharmacology, 395(2), 95-101. https://doi.org/10.1016/S0014-2999(00)00254-5