Information transfer between duplicated chromosomal sequences in mammalian cells involves contiguous regions of DNA

Robert (Mike) Liskay, J. L. Stachelek

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We have investigated the nature of information transfer that appears to occur nonreciprocally between duplicated chromosomal sequences in cultured mouse L cells. We have studied gene conversion between two different defective thymidine kinase genes derived from two closely related strains of type 1 herpes simplex virus and that share a silent restriction site polymorphism. Our results demonstrate that this silent site can be coconverted along with the selected mutant sites. The findings are consistent with a mechanism of gene conversion that involves contiguous blocks of DNA differing in length, position, or both. An additional finding is that the products of coconversion events involving the silent site are unequally recovered although the rates of conversion observed at four different selected sites are similar.

Original languageEnglish (US)
Pages (from-to)1802-1806
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number6
StatePublished - 1986
Externally publishedYes

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Gene Conversion
Thymidine Kinase
DNA
Human Herpesvirus 1
Genes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Information transfer between duplicated chromosomal sequences in mammalian cells involves contiguous regions of DNA. / Liskay, Robert (Mike); Stachelek, J. L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 6, 1986, p. 1802-1806.

Research output: Contribution to journalArticle

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