Influenza virus A infection induces interleukin-8 gene expression in human airway epitheial cells

Augustine M.K. Choi, David B. Jacoby

Research output: Contribution to journalArticle

108 Scopus citations

Abstract

To determine the role of the airway epithelial cell in mediating virus-induced inflammation, we infected primary cultures of human airway epithelial cells with human influenza type A/Port Chalmers/72 (H3N2). After two days, the medium was collected for measurement of the chemstactic cytokine interleukin-8 by enzyme-linked immunosorbent assay. The RNA was extracted from the cells for analysis of interleukin-8 mRNA by Northern blot analysis. Interleukin-8 production was more than doubled by viral infection, while interleukin-8 mRNA was increased four-folsd. Thus induction or interleukin-8 gene expression in virus-infected airway epithelium may be an important early step lending to virus-induced airway inflammation.

Original languageEnglish (US)
Pages (from-to)327-329
Number of pages3
JournalFEBS Letters
Volume309
Issue number3
DOIs
StatePublished - Sep 14 1992

Keywords

  • Airway epithelial cell
  • Cytokine
  • Gene expression
  • Inflammation
  • Interleukin-8
  • Virus

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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