Influence of γ′ Fibrinogen Splice Variant on Fibrin Physical Properties and Fibrinolysis Rate

J. P. Collet, C. Nagaswami, David Farrell, G. Montalescot, J. W. Weisel

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Objective-A splice variant of fibrinogen, γ′, has an altered C-terminal sequence in its gamma chain. This γA/γ′ fibrin is more resistant to lysis than γA/γA fibrin. Whether the physical properties of γ′ and γA fibrin may account for the difference in their fibrinolysis rate remains to be established. Methods and Results-Mechanical and morphological properties of cross-linked purified fibrin, including permeability (Ks, in cm2) and clot stiffness (G′, in dyne/cm2), were measured after clotting γA and γ′ fibrinogens (1 mg/mL). γ′/γ′ fibrin displayed a non-significant decrease in the density of fibrin fibers and slightly thicker fibers than γA/γA fibrin (12±2 fiber/10 -3nm3 versus 16±2 fiber/10-3nm 3 and 274±38 nm versus 257±41 nm for γ′/γ′ and γA/γA fibrin, respectively; P = NS). This resulted in a 20% increase of the permeability constant (6.9±1.7 10-9 cm2 versus 5.5 ± 1.9 10 -9 cm2, respectively; P = NS). Unexpectedly, γ′ fibrin was found to be 3-times stiffer than γA fibrin (72.6±2.6 dyne/cm2 versus 25.1±2.3 dyne/cm 2; P

Original languageEnglish (US)
Pages (from-to)382-386
Number of pages5
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume24
Issue number2
DOIs
StatePublished - Feb 2004

Fingerprint

Fibrinolysis
Fibrin
Fibrinogen
Permeability

Keywords

  • Coagulation
  • Fibrin
  • Fibrinogen
  • Fibrinolysis
  • Thrombosis

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Influence of γ′ Fibrinogen Splice Variant on Fibrin Physical Properties and Fibrinolysis Rate. / Collet, J. P.; Nagaswami, C.; Farrell, David; Montalescot, G.; Weisel, J. W.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 24, No. 2, 02.2004, p. 382-386.

Research output: Contribution to journalArticle

Collet, J. P. ; Nagaswami, C. ; Farrell, David ; Montalescot, G. ; Weisel, J. W. / Influence of γ′ Fibrinogen Splice Variant on Fibrin Physical Properties and Fibrinolysis Rate. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2004 ; Vol. 24, No. 2. pp. 382-386.
@article{17c30c89f48844b092264958b37d976b,
title = "Influence of γ′ Fibrinogen Splice Variant on Fibrin Physical Properties and Fibrinolysis Rate",
abstract = "Objective-A splice variant of fibrinogen, γ′, has an altered C-terminal sequence in its gamma chain. This γA/γ′ fibrin is more resistant to lysis than γA/γA fibrin. Whether the physical properties of γ′ and γA fibrin may account for the difference in their fibrinolysis rate remains to be established. Methods and Results-Mechanical and morphological properties of cross-linked purified fibrin, including permeability (Ks, in cm2) and clot stiffness (G′, in dyne/cm2), were measured after clotting γA and γ′ fibrinogens (1 mg/mL). γ′/γ′ fibrin displayed a non-significant decrease in the density of fibrin fibers and slightly thicker fibers than γA/γA fibrin (12±2 fiber/10 -3nm3 versus 16±2 fiber/10-3nm 3 and 274±38 nm versus 257±41 nm for γ′/γ′ and γA/γA fibrin, respectively; P = NS). This resulted in a 20{\%} increase of the permeability constant (6.9±1.7 10-9 cm2 versus 5.5 ± 1.9 10 -9 cm2, respectively; P = NS). Unexpectedly, γ′ fibrin was found to be 3-times stiffer than γA fibrin (72.6±2.6 dyne/cm2 versus 25.1±2.3 dyne/cm 2; P",
keywords = "Coagulation, Fibrin, Fibrinogen, Fibrinolysis, Thrombosis",
author = "Collet, {J. P.} and C. Nagaswami and David Farrell and G. Montalescot and Weisel, {J. W.}",
year = "2004",
month = "2",
doi = "10.1161/01.ATV.0000109748.77727.3e",
language = "English (US)",
volume = "24",
pages = "382--386",
journal = "Arteriosclerosis, Thrombosis, and Vascular Biology",
issn = "1079-5642",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Influence of γ′ Fibrinogen Splice Variant on Fibrin Physical Properties and Fibrinolysis Rate

AU - Collet, J. P.

AU - Nagaswami, C.

AU - Farrell, David

AU - Montalescot, G.

AU - Weisel, J. W.

PY - 2004/2

Y1 - 2004/2

N2 - Objective-A splice variant of fibrinogen, γ′, has an altered C-terminal sequence in its gamma chain. This γA/γ′ fibrin is more resistant to lysis than γA/γA fibrin. Whether the physical properties of γ′ and γA fibrin may account for the difference in their fibrinolysis rate remains to be established. Methods and Results-Mechanical and morphological properties of cross-linked purified fibrin, including permeability (Ks, in cm2) and clot stiffness (G′, in dyne/cm2), were measured after clotting γA and γ′ fibrinogens (1 mg/mL). γ′/γ′ fibrin displayed a non-significant decrease in the density of fibrin fibers and slightly thicker fibers than γA/γA fibrin (12±2 fiber/10 -3nm3 versus 16±2 fiber/10-3nm 3 and 274±38 nm versus 257±41 nm for γ′/γ′ and γA/γA fibrin, respectively; P = NS). This resulted in a 20% increase of the permeability constant (6.9±1.7 10-9 cm2 versus 5.5 ± 1.9 10 -9 cm2, respectively; P = NS). Unexpectedly, γ′ fibrin was found to be 3-times stiffer than γA fibrin (72.6±2.6 dyne/cm2 versus 25.1±2.3 dyne/cm 2; P

AB - Objective-A splice variant of fibrinogen, γ′, has an altered C-terminal sequence in its gamma chain. This γA/γ′ fibrin is more resistant to lysis than γA/γA fibrin. Whether the physical properties of γ′ and γA fibrin may account for the difference in their fibrinolysis rate remains to be established. Methods and Results-Mechanical and morphological properties of cross-linked purified fibrin, including permeability (Ks, in cm2) and clot stiffness (G′, in dyne/cm2), were measured after clotting γA and γ′ fibrinogens (1 mg/mL). γ′/γ′ fibrin displayed a non-significant decrease in the density of fibrin fibers and slightly thicker fibers than γA/γA fibrin (12±2 fiber/10 -3nm3 versus 16±2 fiber/10-3nm 3 and 274±38 nm versus 257±41 nm for γ′/γ′ and γA/γA fibrin, respectively; P = NS). This resulted in a 20% increase of the permeability constant (6.9±1.7 10-9 cm2 versus 5.5 ± 1.9 10 -9 cm2, respectively; P = NS). Unexpectedly, γ′ fibrin was found to be 3-times stiffer than γA fibrin (72.6±2.6 dyne/cm2 versus 25.1±2.3 dyne/cm 2; P

KW - Coagulation

KW - Fibrin

KW - Fibrinogen

KW - Fibrinolysis

KW - Thrombosis

UR - http://www.scopus.com/inward/record.url?scp=1042302749&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1042302749&partnerID=8YFLogxK

U2 - 10.1161/01.ATV.0000109748.77727.3e

DO - 10.1161/01.ATV.0000109748.77727.3e

M3 - Article

VL - 24

SP - 382

EP - 386

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

SN - 1079-5642

IS - 2

ER -