Abstract
Objective: To evaluate the effect of pathological placental conditions such as intrauterine growth restriction (IUGR) or exposure to angiotensin II (AII) on TNF-α secretion in the vasculature of isolated human placental cotyledons. Study design: Isolated placental cotyledons from 10 normal and four intrauterine growth restricted fetuses were dually perfused. Perfusate samples from the fetal circulation were collected every 30 min during 120 min. TNF-α levels in the fetal-placental perfusate were evaluated using specific commercial ELISA kits. In three additional normal placentae, bolus injections of angiotensin II (10 -9 -10 -4 mol/l) were given into the fetal-placental circulation and perfusate samples were collected. Statistical significance of difference TNF-α levels between different conditions was determined by analysis of variance (ANOVA) and paired t-test. Results: TNF-α levels were significantly higher in the perfusate of IUGR placentae as compared with normal placentae after 120 min of perfusion (mean 410±121 vs. 39±14 pg/ml, P = 0.005). There was a significant dose-dependent increase in TNF-α levels in the placental perfusate after a bolus injection of AII 66 pg/ml with AII 10 -9 mol/l vs. 97 pg/ml with AII 10 -5 mol/l (P = 0.004), respectively. Conclusions: Placental pathology related to condition IUGR might induce the secretion of proinflammatory cytokines such as TNF-α, which may enhance the vasoconstriction of the fetal placental vascular bed.
Original language | English (US) |
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Pages (from-to) | 69-72 |
Number of pages | 4 |
Journal | European Journal of Obstetrics and Gynecology and Reproductive Biology |
Volume | 94 |
Issue number | 1 |
DOIs | |
State | Published - 2001 |
Externally published | Yes |
Keywords
- Angiotensin II
- IUGR
- Inflammatory cytokines
- Placenta
- Tumor necrosis factor
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynecology