Increased oxygen exposure alters collagen expression and tissue architecture during ligature-induced periodontitis

P. K. Gajendrareddy, R. Junges, G. Cygan, Y. Zhao, P. T. Marucha, C. G. Engeland

Research output: Research - peer-reviewArticle

Abstract

Background and Objective: The aim of this study was to evaluate the effects of increased oxygen availability on gene expression and on collagen deposition/maturation in the periodontium following disease. Material and Methods: Male Wistar rats had ligatures placed around their molars to induce periodontal disease, and a subset of animals underwent hyperbaric oxygen (HBO) treatment for 2 h twice per day. At 15 and 28 d, tissue gene expression of COL1A1, transforming growth factor-β1 and alkaline phosphatase was determined; other histological samples were stained with Picrosirius red to evaluate levels of collagen deposition, maturation and thickness. Results: In animals that underwent HBO treatment, type I collagen expression was higher and collagen deposition, maturation and thickness were more robust. Reduced mRNA levels of transforming growth factor-beta1 and alkaline phosphatase in HBO-treated rats on day 28 suggested that a quicker resolution in both soft tissue and bone remodeling occurred following oxygen treatment. No differences in inflammation were observed between groups. Conclusions: The extracellular matrix regenerated more quickly in the HBO-treated group as evidenced by higher collagen expression, deposition and maturation.

LanguageEnglish (US)
JournalJournal of Periodontal Research
DOIs
StateAccepted/In press - 2016

Fingerprint

Periodontitis
Ligation
Collagen
Oxygen
Alkaline Phosphatase
Gene Expression
Transforming Growth Factor beta1
Periodontium
Bone Remodeling
Transforming Growth Factors
Periodontal Diseases
Collagen Type I
Extracellular Matrix
Wistar Rats
Inflammation
Messenger RNA
C.I. direct red 80

Keywords

  • Alkaline phosphatase
  • Collagen type I
  • Cytokine
  • Hyperbaric oxygen
  • Inflammation
  • Transforming growth factor beta1

ASJC Scopus subject areas

  • Periodontics

Cite this

Increased oxygen exposure alters collagen expression and tissue architecture during ligature-induced periodontitis. / Gajendrareddy, P. K.; Junges, R.; Cygan, G.; Zhao, Y.; Marucha, P. T.; Engeland, C. G.

In: Journal of Periodontal Research, 2016.

Research output: Research - peer-reviewArticle

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AU - Cygan,G.

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AU - Marucha,P. T.

AU - Engeland,C. G.

PY - 2016

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N2 - Background and Objective: The aim of this study was to evaluate the effects of increased oxygen availability on gene expression and on collagen deposition/maturation in the periodontium following disease. Material and Methods: Male Wistar rats had ligatures placed around their molars to induce periodontal disease, and a subset of animals underwent hyperbaric oxygen (HBO) treatment for 2 h twice per day. At 15 and 28 d, tissue gene expression of COL1A1, transforming growth factor-β1 and alkaline phosphatase was determined; other histological samples were stained with Picrosirius red to evaluate levels of collagen deposition, maturation and thickness. Results: In animals that underwent HBO treatment, type I collagen expression was higher and collagen deposition, maturation and thickness were more robust. Reduced mRNA levels of transforming growth factor-beta1 and alkaline phosphatase in HBO-treated rats on day 28 suggested that a quicker resolution in both soft tissue and bone remodeling occurred following oxygen treatment. No differences in inflammation were observed between groups. Conclusions: The extracellular matrix regenerated more quickly in the HBO-treated group as evidenced by higher collagen expression, deposition and maturation.

AB - Background and Objective: The aim of this study was to evaluate the effects of increased oxygen availability on gene expression and on collagen deposition/maturation in the periodontium following disease. Material and Methods: Male Wistar rats had ligatures placed around their molars to induce periodontal disease, and a subset of animals underwent hyperbaric oxygen (HBO) treatment for 2 h twice per day. At 15 and 28 d, tissue gene expression of COL1A1, transforming growth factor-β1 and alkaline phosphatase was determined; other histological samples were stained with Picrosirius red to evaluate levels of collagen deposition, maturation and thickness. Results: In animals that underwent HBO treatment, type I collagen expression was higher and collagen deposition, maturation and thickness were more robust. Reduced mRNA levels of transforming growth factor-beta1 and alkaline phosphatase in HBO-treated rats on day 28 suggested that a quicker resolution in both soft tissue and bone remodeling occurred following oxygen treatment. No differences in inflammation were observed between groups. Conclusions: The extracellular matrix regenerated more quickly in the HBO-treated group as evidenced by higher collagen expression, deposition and maturation.

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