Increased expression of basic fibroblast growth factor in hyperoxic-injured mouse lung

Research output: Contribution to journalArticle

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Abstract

Basic fibroblast growth factor (bFGF) is a mitogenic polypeptide for a wide variety of cell types and has been immunolocalized in the rodent and human lung. We investigated the mRNA and protein expression of bFGF in hyperoxic-injured adult mouse lungs using northern blot analysis and immunohistochemistry. Mice (6-8 weeks) were continuously exposed to 80% oxygen up to 4 days. Levels of bFGF mRNA were increased from room air control on days 3 and 4 of hyperoxia. mRNA levels of acidic fibroblast growth factor (aFGF), fibronectin, and transin/stromelysin were also examined in this injury model. Similar to bFGF, the fibronectin and transin/stromelysin mRNA levels were increased after 3 days of hyperoxia. In contrast, the aFGF mRNA levels were gradually reduced on each day of hyperoxia. A rabbit polyclonal anti-bFGF antibody was used to determine the distribution and levels of expression in the hyperoxic-injured lungs. The room air control and day 1 hyperoxic-exposed lungs exhibited staining for bFGF in the basement membranes of the blood vessels, airways, and alveoli. Patchy but intense alveolar staining was prominent on day 4 of hyperoxia. The bFGF immunoreactivity of blood vessels and airways was unaffected by the hyperoxia exposure. These results suggest that bFGF may play a role in the alveolar response to hyperoxic-induced injury by virtue of the altered mRNA levels and protein distribution in this injury model.

Original languageEnglish (US)
Pages (from-to)536-543
Number of pages8
JournalJournal of Cellular Biochemistry
Volume56
Issue number4
DOIs
StatePublished - 1994

Fingerprint

Fibroblast Growth Factor 2
Hyperoxia
Matrix Metalloproteinase 3
Lung
Messenger RNA
Fibroblast Growth Factor 1
Blood vessels
Fibronectins
Blood Vessels
Wounds and Injuries
Air
Staining and Labeling
Basement Membrane
Northern Blotting
Rodentia
Proteins
Immunohistochemistry
Oxygen
Rabbits
Peptides

Keywords

  • Acute lung injury
  • Basic fibroblast growth factor
  • Fibronectin
  • Stromelysin
  • Transin

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Increased expression of basic fibroblast growth factor in hyperoxic-injured mouse lung. / Powers, Michael (Mike); Planck, Stephen; Berger, J.; Wall, Michael; Rosenbaum, James (Jim).

In: Journal of Cellular Biochemistry, Vol. 56, No. 4, 1994, p. 536-543.

Research output: Contribution to journalArticle

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