Increased calpain correlates with Th1 cytokine profile in PBMCs from MS patients

Sarah A. Imam, Mary K. Guyton, Azizul Haque, Arthur Vandenbark, William R. Tyor, Swapan K. Ray, Naren L. Banik

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Multiple sclerosis (MS) is a devastating autoimmune demyelinating disease of the central nervous system (CNS). This study investigated whether expression and activity of the calcium-activated protease calpain correlated with Th1/Th2 dysregulation in MS patients during states of relapse and remission. Calpain expression and activity were significantly increased in peripheral blood mononuclear cells (PBMCs) from MS patients, compared to controls, with the highest expression and activity noted during relapse. Th1 cytokines were highest and Th2 cytokines were lowest in MS patients during relapse. Treatment with calpain inhibitor, calpeptin, decreased Th1 cytokines in PBMCs from MS patients. Calpain inhibitor also reduced degradation of myelin basic protein (MBP) by inhibiting the calpain secreted from MBP-specific T cells. Taken together, these results suggested calpain involvement in Th1/Th2 dysregulation in MS patients.

Original languageEnglish (US)
Pages (from-to)139-145
Number of pages7
JournalJournal of Neuroimmunology
Volume190
Issue number1-2
DOIs
StatePublished - Oct 2007

Keywords

  • Calpain
  • Cytokines
  • Multiple sclerosis
  • T cell activation
  • Th1/Th2 dysregulation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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