In vivo nerve-specificity of rhodamines and Si-rhodamines

Antonio R. Montano, Lei G. Wang, Connor W. Barth, Nourhan A. Shams, K. A.Sashini U. Kumarapeli, Summer L. Gibbs

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Accidental nerve damage or transection of vital nerve structures remains an unfortunate reality that is often aßociated with surgery. Despite the existence of nerve-sparing techniques, the succeß of such procedures is not only complicated by anatomical variance acroß patients but is also highly dependent on a surgeon's first-hand experience that is acquired over numerous procedures through trial and error, often with highly variable succeß rates. Fluorescent small molecules, such as rhodamines and fluoresceins have proven incredibly useful for biological imaging in the life sciences, and they appeared to have potential in illuminating vital nerve structures during surgical procedures. In order to make use of the current clinically relevant imaging systems and to provide surgeons with fluorescent contrast largely free from the interference of hemoglobin and water, it was first neceßary to spectrally tune known fluorescent scaffolds towards near infrared (NIR) wavelengths. To determine whether the well-documented Si-substitution strategy could be applied towards developing a NIR fluorophore that retained nerve-specific properties of candidate molecules, an in vivo comparison was made between two compounds previously shown to highlight nervous structures - TMR and Rhodamine B - and their Si-substituted derivatives.

Original languageEnglish (US)
Title of host publicationMolecular-Guided Surgery
Subtitle of host publicationMolecules, Devices, and Applications VI
EditorsSylvain Gioux, Summer L. Gibbs, Brian W. Pogue
PublisherSPIE
ISBN (Electronic)9781510632073
DOIs
StatePublished - Jan 1 2020
EventMolecular-Guided Surgery: Molecules, Devices, and Applications VI 2020 - San Francisco, United States
Duration: Feb 1 2020Feb 2 2020

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11222
ISSN (Print)1605-7422

Conference

ConferenceMolecular-Guided Surgery: Molecules, Devices, and Applications VI 2020
CountryUnited States
CitySan Francisco
Period2/1/202/2/20

Keywords

  • Contrast agent
  • Fluorescent imaging
  • Image-guided surgery
  • Nerve-specific Fluorophores

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Montano, A. R., Wang, L. G., Barth, C. W., Shams, N. A., Kumarapeli, K. A. S. U., & Gibbs, S. L. (2020). In vivo nerve-specificity of rhodamines and Si-rhodamines. In S. Gioux, S. L. Gibbs, & B. W. Pogue (Eds.), Molecular-Guided Surgery: Molecules, Devices, and Applications VI [112220I] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11222). SPIE. https://doi.org/10.1117/12.2545311