In-vivo degradation of polyurethanes: Transmission-FTIR microscopic characterization of polyurethanes sectioned by cryomicrotomy

Simon McCarthy, Gordon F. Meijs, Natasha Mitchell, Pathiraja A. Gunatillake, Graeme Heath, Arthur Brandwood, Klaus Schindhelm

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

A combination of cryomicrotomy and transmission Fourier transform infrared (FTIR) microscopy was used to investigate chemical changes in unstrained sheets of Pellethane® 2363-80A, Tecoflex® EG80A and Biomer® caused by biodegradation (18 month subcutaneous ovine implant). Cryomicrotomy was used to obtain thin sections (ca. 2.5 μm) from the surface into the bulk, parallel to the plane of the surface. FTIR microscopy was then used to obtain infrared absorbance spectra in the range 4000-600 cm-1. Comparisons between the infrared spectra (by spectral subtraction) from implant surface, implant interior and non-implanted controls were used to detect chemical changes. Scanning electron microscopy was used to assess microstructural changes owing to biodegradation. Biodegradation in Biomer® was observed as uniform pitting and superficial fissuring (<2.0 μm depth) over the implant surface. Biodegradation in Pellethane® 2363-80A and Tecoflex® EG80A was observed as severe localized embrittlement of the surface with fissures infiltrating up to 40 μm into the bulk. The chemical changes associated with biodegradation were observed as localized oxidation of the soft segment and hydrolysis of the urethane bonds joining hard and soft segments. Tecoflex® EG80A was also found to be susceptible to localized hydrolysis of the urethane bond within the aliphatic hard segment. Biomer® showed evidence of a significant non-specific degradation in the non-implanted wet control (37°C phosphate buffered saline at pH 7.3) samples and in the implant bulk.

Original languageEnglish (US)
Pages (from-to)1387-1409
Number of pages23
JournalBiomaterials
Volume18
Issue number21
DOIs
StatePublished - Nov 1997
Externally publishedYes

Fingerprint

Polyurethanes
Fourier Analysis
Biodegradation
Fourier transforms
Urethane
Infrared radiation
Degradation
Microscopy
Hydrolysis
Microscopic examination
Electron Scanning Microscopy
Sheep
Phosphates
Embrittlement
Pitting
Joining
Tecoflex
biomer
Oxidation
Scanning electron microscopy

Keywords

  • Biodegradation
  • Cryomicrotomy
  • FTIR-microscopy
  • Polyurethane

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

McCarthy, S., Meijs, G. F., Mitchell, N., Gunatillake, P. A., Heath, G., Brandwood, A., & Schindhelm, K. (1997). In-vivo degradation of polyurethanes: Transmission-FTIR microscopic characterization of polyurethanes sectioned by cryomicrotomy. Biomaterials, 18(21), 1387-1409. https://doi.org/10.1016/S0142-9612(97)00083-5

In-vivo degradation of polyurethanes : Transmission-FTIR microscopic characterization of polyurethanes sectioned by cryomicrotomy. / McCarthy, Simon; Meijs, Gordon F.; Mitchell, Natasha; Gunatillake, Pathiraja A.; Heath, Graeme; Brandwood, Arthur; Schindhelm, Klaus.

In: Biomaterials, Vol. 18, No. 21, 11.1997, p. 1387-1409.

Research output: Contribution to journalArticle

McCarthy, S, Meijs, GF, Mitchell, N, Gunatillake, PA, Heath, G, Brandwood, A & Schindhelm, K 1997, 'In-vivo degradation of polyurethanes: Transmission-FTIR microscopic characterization of polyurethanes sectioned by cryomicrotomy', Biomaterials, vol. 18, no. 21, pp. 1387-1409. https://doi.org/10.1016/S0142-9612(97)00083-5
McCarthy, Simon ; Meijs, Gordon F. ; Mitchell, Natasha ; Gunatillake, Pathiraja A. ; Heath, Graeme ; Brandwood, Arthur ; Schindhelm, Klaus. / In-vivo degradation of polyurethanes : Transmission-FTIR microscopic characterization of polyurethanes sectioned by cryomicrotomy. In: Biomaterials. 1997 ; Vol. 18, No. 21. pp. 1387-1409.
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