In vitro antibody synthesis in 20 μl hanging drops: Initiation of secondary responses and a simple method of cloning hybridomas

Eric B. Bell, McKay Brown, Marvin B. Rittenberg

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

An in vitro culture system is described in which antibody synthesis is initiated by 105 hapten-primed spleen cells in 20 μl hanging drops. Plaque-forming cell (PFC) responses to thymus dependent and thymus independent antigens were obtained in drop cultures comparable to the micro Mishell-Dutton system. Antigens could be administered in soluble form or on antigen-pulsed peritoneal cells. The hanging drop method also provides a reliable yet simple procedure of cloning hybridoma cells while permitting direct visualization of the number of cells in each drop.

Original languageEnglish (US)
Pages (from-to)137-145
Number of pages9
JournalJournal of Immunological Methods
Volume62
Issue number2
DOIs
StatePublished - Aug 26 1983

Keywords

  • cell cloning
  • hanging drop cultures
  • hybridomas
  • in vitro antibody synthesis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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